中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (39): 7288-7291.doi: 10.3969/j.issn.1673-8225.2011.39.017

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

快速建立小牛椎体骨质疏松模型

曹  涌,张  烽,姚  羽,邱  军,陈向东,赵  剑   

  1. 南通大学附属医院脊柱外科,江苏省南通市226001
  • 收稿日期:2011-05-27 修回日期:2011-08-17 出版日期:2011-09-24
  • 通讯作者: 张烽,教授,博士,主任医师,硕士生导师,南通大学附属医院脊柱外科,江苏省南通市226001
  • 作者简介:曹涌★,男,1971年生,江苏省南通市人,汉族,南通大学骨外科学毕业,硕士,副主任医师,主要从事脊柱外科研究。 yaoyu1122@sina.com 并列第一作者:姚羽,男,1981年生,江苏省南通市人,汉族,南通大学骨外科学毕业,硕士,医师,主要从事脊柱外科研究。 yaoyu1122@sina.com

Rapid establishment of a calf vertebral osteoporosis model

Cao Yong, Zhang Feng, Yao Yu, Qiu Jun, Chen Xiang-dong, Zhao Jian   

  1. Orthopedics Department, Affiliated Hospital of Nantong University, Nantong  226001, Jiangsu Province, China
  • Received:2011-05-27 Revised:2011-08-17 Online:2011-09-24
  • Contact: Zhang Feng, Doctor, Professor, Chief physician, Orthopedics Department, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China
  • About author:Cao Yong★, Master, Associate chief physician, Orthopedics Department, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China yaoyu1122@sina.com Yao Yu, Master, Physician, Orthopedics Department, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China yaoyu1122@sina.com Cao Yong and Yao Yu contributed equally to this paper.

摘要:

背景:目前用于脊柱生物力学研究的骨质疏松动物模型是以去势雌性动物或服药动物为代表的动物模型,但存在建立时间长、可靠性差的问题。
目的:利用Shandon TBD-1脱钙法快速建立牛椎体骨质疏松模型。
方法: 选取新鲜小牛脊柱第6~11节椎体,共24个椎体,测量骨密度后,随机分为3组:预实验组采用Shandon TBD-1浸泡椎体,脱钙组将Shandon TBD-1注入椎体一侧钉眼内,对照组不做脱钙处理。脱钙后再次测量骨密度,将相同规格的椎弓根螺钉拧入双侧椎弓根,测试其最大轴向拔出力。
结果与结论:预实验组椎体脱钙后椎体骨密度随着脱钙时间的延长而降低;脱钙组骨密度值、椎弓根螺钉最大轴向拔出力均低于对照组( P < 0.01),且骨密度与椎弓根螺钉最大轴向拔出力存在线性正相关。说明应用Shandon TBD-1脱钙剂对小牛椎体脱钙是一种快速制备骨质疏松动物模型的方法,并能够很好模拟骨质疏松的力学性能。

关键词: 骨质疏松, 脱钙骨体外模, 生物力学, 骨密度, 动物模型

Abstract:

BACKGROUND: Currently, osteoporosis animal models used for biomechanical studies of spinal osteoporosis are ovariectomized animal models or drug-induced animal models, and they have many problems such as model establishment for a long time and poor reliability.
OBJECTIVE: To establish an animal model of osteoporotic vertebras rapidly by decalcifying fresh calf lumbar vertebras with Shandon TBD-1.
METHODS: Twenty-four calf lumbar vertebrae from four fresh calves were classified into three groups: in pre-experiment group, the vertebrae were immersed in Shandon TBD-1; in decalcified group, the vertebrae were decalcified by using Shandon TBD-1; in control group, no decalcification was done. The vertebrae were subjected to DEXA to measure bone mineral density (BMD). And then two pedicle screws were driven into calf lumbar vertebrae. After that, the maximum axial pullout strength was recorded.
RESULTS AND CONCLUSION: The BMD of the vertebrae in the pre-experiment group was decreased with the time of decalcification. The BMD and the maximum axial pullout strength in the decalcified group were significantly lower than those in the control group (P < 0.01), and there was a linear positive correlation between the BMD and the maximum axial pullout strength. An animal model of osteoporotic vertebra can be established rapidly in calf by means of decalcifying fresh calf lumbar vertebras with Shandon TBD-1.

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