中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (11): 1910-1914.doi: 10.3969/j.issn.1673-8225.2012.11.003

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

骨形态发生蛋白与碱性成纤维细胞生长因子联合修复软骨缺损的效果评价*★

朱国华1,蔡建平1,郭翠玲1,廖家新1,刘  勇1,罗洪涛1,许国华2,胡红涛2   

  1. 1南京中医药大学无锡附属医院骨伤研究所,江苏省无锡市 214001;   2解放军第二军医大学长征医院骨科,上海市 200003
  • 收稿日期:2011-07-09 修回日期:2011-07-20 出版日期:2012-03-11
  • 作者简介:朱国华★,男,1969年生,江苏省兴化市人,汉族,硕士,副主任医师,主要从事骨伤科基础与临床研究。 zhuguohuazhu@163.com
  • 基金资助:

    江苏省无锡市科技局科技发展计划项目(No. CSZ00857),骨软骨移植结合细胞生长因子修复关节软骨缺损。

Effects of recombinant human bone morphogenetic protein combined with basic fibroblast growth factor on the repair of articular cartilage defects

Zhu Guo-hua1, Cai Jian-ping1, Guo Cui-ling1, Liao Jia-xin1, Liu Yong1, Luo Hong-tao1, Xu Guo-hua2, Hu Hong-tao2   

  1. 1Institute of Orthopedics, Wuxi Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Wuxi  214001, Jiangsu Province, China;    2Institute of Orthopedics, Changzheng Hospital, the Second Military Medical University of Chinese PLA, Shanghai  200003, China
  • Received:2011-07-09 Revised:2011-07-20 Online:2012-03-11
  • About author:Zhu Guo-hua★, Master, Associate chief physician, Institute of Orthopedics, Wuxi Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Wuxi 214001, Jiangsu Province, China zhuguohuazhu@163.com
  • Supported by:

    Program of Science and Technology of Wuxi Technology Bureau in Jiangsu Province, No. CSZ00857*

摘要:

背景:多种细胞生长因子在骨软骨代谢过程中的协同作用越来越受到重视,但目前复合细胞生长因子修复软骨缺损报道较少,且修复效果尚无定论。
目的:探讨骨形态发生蛋白和碱性成纤维细胞生长因子联合应用修复关节软骨缺损的效果。
方法:24只日本大耳白兔建立骨软骨缺损模型后随机等分为4组,对照组缺损处仅填塞明胶海绵,其他3组在对照组基础上,缺损处分别注射骨形态发生蛋白和碱性成纤维细胞生长因子、骨形态发生蛋白、碱性成纤维细胞生长因子。
结果与结论:大体观察显示联合应用2种细胞因子后,软骨缺损面基本修复但稍不平整,单独使用其中1种细胞因子缺损面未完全修复,对照组无明显修复。联合应用2种细胞因子缺损部位软骨细胞数多于其他3组(P < 0.05),且Ⅱ型胶原免疫组化染色深于其他组。提示联合应用骨形态发生蛋白和碱性成纤维细胞生长因子可以促进关节软骨损伤的修复,疗效优于单独应用骨形态发生蛋白或碱性成纤维细胞生长因子。
关键词:骨形态发生蛋白;碱性成纤维细胞生长因子;修复;软骨缺损;细胞因子
doi:10.3969/j.issn.1673-8225.2012.11.003

关键词: 骨形态发生蛋白, 碱性成纤维细胞生长因子, 修复, 软骨缺损, 细胞因子

Abstract:

BACKGROUND: The synergy of various cell growth factors attracts more and more attention in the course of cartilage metabolism. However, there are few reports of repairing cartilage defects with combined cell growth factors, and the effect remains unknown at present.
OBJECTIVE: To study the repairing effect of recombinant human bone morphogenetic protein (rhBMP) combined with basic fibroblast growth factor (bFGF) on articular cartilage defects.
METHODS: After the model of articular cartilage defects was made, 24 Japan big-eared white rabbits were randomly divided into four groups for intervention: rhBMP combined with bFGF (group A), single rhBMP (group B), single bFGF (group C), the fourth group was without injection and just filled with gelatin sponge (group D).
RESULTS AND CONCLUSION: In general observation, articular cartilage defects were basically repaired but slightly uneven in group A, and there were no defects entirely repaired in groups B and C. In group D, articular cartilage defects were not repaired. The number of cartilage cells in group A was more than that in groups B, C and D (P < 0.05). The positive number of type Ⅱ collagens by immunohistochemical staining in group A was higher than that in the other groups. It indicated that rhBMP combined with bFGF could accelerate the formation of new articular cartilage, and had better effect than rhBMP or bFGF alone. 

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