中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (11): 1919-1922.doi: 10.3969/j.issn.1673-8225.2012.11.005

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

碱性成纤维细胞生长因子对乙醇中毒大鼠ATP酶及抗氧化作用的影响*★

黄俊杰,王彩冰,何显教,黄丽娟,黄彦峰,梁祚仁,阳秀英,黎  昀,赵善民   

  1. 右江民族医学院应用生理研究室,广西壮族自治区百色市  533000
  • 收稿日期:2011-08-27 修回日期:2011-11-12 出版日期:2012-03-11
  • 作者简介:黄俊杰★,男,1970年生,广西壮族自治区平果县人,壮族,2007年广西医科大学毕业,硕士,副教授,主要从事神经生理与神经疾病方面的研究。moke68@163.com
  • 基金资助:

    广西教育厅科研项目(200810LX045)。

Effects of basic fibroblast growth factor on adenosine triphosphatase activity and antioxidation of alcoholism rat models   

Huang Jun-jie, Wang Cai-bing, He Xian-jiao, Huang Li-juan, Huang Yan-feng, Liang Zuo-ren, Yang Xiu-ying, Li Yun, Zhao Shan-min   

  1. Department of Applied Physiology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China
  • Received:2011-08-27 Revised:2011-11-12 Online:2012-03-11
  • About author:Huang Jun-jie★, Master, Associate professor, Department of Applied Physiology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China moke68@163.com
  • Supported by:

    Research Foundation of Guangxi Education Bureau, No. 200810LX045*

摘要:

背景:碱性成纤维细胞生长因子具有多种生物活性,对组织创伤具有修复作用,但其对乙醇中毒的保护作用至今未见报道。
目的:观察碱性成纤维细胞生长因子对乙醇中毒大鼠脑和肝组织中ATP酶、超氧化物歧化酶活力和丙二醛水平的影响。
方法:选择成年Wistar雄性大鼠,采用白酒灌胃建立乙醇中毒模型,30只造模成功的大鼠分为3组,生理盐水组和碱性成纤维细胞生长因子治疗组分别于造模60 d后肌肉注射生理盐水和碱性成纤维细胞生长因子;中毒模型组不作任何干预;另取10只不灌白酒作为正常对照组。
结果与结论:与正常对照组相比,乙醇中毒后大鼠肝及脑组织ATP酶活力和超氧化物歧化酶活力显著降低,丙二醛水平明显升高(P < 0.05);用碱性成纤维细胞生长因子治疗后ATP酶活力和超氧化物歧化酶活力均显著升高,丙二醛水平显著降低(P < 0.05~0.01)。提示碱性成纤维细胞生长因子能提高ATP酶活力且有抗氧化作用,对乙醇中毒所致的脑和肝损伤具有保护作用。
关键词:碱性成纤维细胞生长因子;酒精中毒;ATP酶;超氧化物歧化酶;丙二醛
doi:10.3969/j.issn.1673-8225.2012.11.005 

关键词: 碱性成纤维细胞生长因子, 酒精中毒, ATP酶, 超氧化物歧化酶, 丙二醛

Abstract:

BACKGROUND: Basic fibroblast growth factor (bFGF) has multiple biological activities, and has positive effect on the recovery of tissue trauma. But the effect of bFGF against alcoholism has not been reported.
OBJECTIVE: To study the effect of bFGF on the activities of adenosine triphosphatase (ATPase) and superoxide dismutase (SOD) and malondialdehyde (MDA) in cerebral cortex and liver tissue of alcoholism rat model.
METHODS: The alcoholism rat models were established by perfusing stomach with alcohol. Thirty Wistar rats were randomly divided into three groups. Normal saline group and bFGF treatment group were injected with normal saline and bFGF, respectively, at 60 days after modeling; Alcoholism model group was treated with no intervention. Another 10 rats without alcohol served as control group.
RESULTS AND CONCLUSION: The activities of ATPase and SOD in the cerebral cortex and liver tissue of alcoholism model group were significantly decreased than those in the control group, but the content of MDA was significantly higher than that in the control group (P < 0.05). After bFGF intervention, the activities of ATPase and SOD in the cerebral cortex and liver tissue were significantly increased compared with the normal saline group and alcoholism model group, but the content of MDA was significantly decreased (P < 0.05-0.01). bFGF can increase the activities of ATPase and the antioxidative ability of brain tissue in alcoholism model rats. The bFGF can protect the brain and liver of rats against alcoholism.
 

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