中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (11): 1923-1926.doi: 10.3969/j.issn.1673-8225.2012.11.006

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

肾间质纤维化模型大鼠转化生长因子β/Smads信号传导通路变化与温阳活血方的干预**★

胡绍进1,李  均2   

  1. 1湖南省邵阳市中心医院康复科,湖南省邵阳市   422000;2遵义医学院第五附属医院中医科,广东省珠海市   519000
  • 收稿日期:2011-07-08 修回日期:2011-10-19 出版日期:2012-03-11
  • 通讯作者: 李均,博士,主任医师,硕士生导师,遵义医学院第五附属医院中医科,广东省珠海市 519000 lijun69-1214@163.com
  • 作者简介:胡绍进★,男,1969年生,湖南省辰溪县人,汉族,2009年遵义医学院毕业,硕士,主要从事内科康复研究。 Hushaojin2006@yahoo.com.cn
  • 基金资助:

    贵州省省长基金资助项目(C-263);遵义医学院博士启动基金资助课题(2004-007)。

Effects of Wenyang Huoxue recipe on transforming growth factor beta/Smad signal transduction pathway in model rats of renal interstitial fibrosis 

Hu Shao-jin1, Li Jun2   

  1. 1Department of Rehabilitation, the Central Hospital of Shaoyang, Shaoyang  422000, Hunan Province, China; 2Department of Traditional Chinese Medicine, Fifth Affiliated Hospital of Zunyi Medical College, Zhuhai  519000,  Guangdong Province, China
  • Received:2011-07-08 Revised:2011-10-19 Online:2012-03-11
  • Contact: author: Li Jun, Doctor, Chief physician, Master’s supervisor, Department of Traditional Chinese Medicine, Fifth Affiliated Hospital of Zunyi Medical College, Zhuhai 519000, Guangdong Province, China lijun69-1214@163.com
  • About author:Hu Shao-jin★, Master, Department of Rehabilitation, the Central Hospital of Shaoyang, Shaoyang 422000, Hunan Province, China Hushaojin2006@yahoo.com.cn
  • Supported by:

    the Foundation of Governor of Guizhou Province, No. C-263*; the Staring Foundation for Doctors of Zunyi Medical College, No.2004-007*

摘要:

背景:转化生长因子β/Smad信号转导通路在肾纤维化的发生发展中起重要作用。
目的:探讨温阳活血方对肾纤维化转化生长因子β/Smads信号传导通路的影响。
方法:选用清洁级雄性SD大鼠28只,随机分为假手术组和模型组,模型组行左侧输尿管结扎术,单侧输尿管梗阻模型。假手术组大鼠打开腹腔后分离左侧输尿管但不结扎,随即关闭腹腔。造模后第2天开始分别给予温阳活血方、血管紧张素转化酶抑制剂或生理盐水灌胃,1次/d,各组均连续灌胃14 d。
结果与结论:温阳活血组转化生长因子β1、Smads3的表达显著低于模型组(P < 0.01), Smad7表达显著高于模型组       (P < 0.01),与福辛普利组相当。推测温阳活血方可能通过抑制转化生长因子β1、Smads3的表达,上调Smad7的表达,进而影响转化生长因子β/Smad信号传导通路,对肾间质纤维化起防治作用。

关键词: 肾间质纤维化模型, 温阳活血, 转化生长因子&beta, 1, Smads3, Smad7

Abstract:

BACKGROUND: Transforming growth factor β/Smad signal transduction pathway plays an important role in the occurrence and development of renal fibrosis.
OBJECTIVE: To investigate the effects of Wenyang Huoxue recipe on transforming growth factor β/Smad signal transduction pathway in renal interstitial fibrosis rats.
METHODS: A total of 28 male SD rats of clean grade were randomly assigned into sham operation group and model group. Left urethral ligation was performed in rats to construct unilateral ureteral obstruction model. The left ureter of sham operation rats was isolated without ligation after opening the abdominal cavity; then the abdominal cavity was closed. The rats were administrated with Wenyang Huoxue recipe by gavage once. Rats in both groups were administered by gavage for 14 days. RESULTS AND CONCLUSION: The expression of transforming growth factor β1 and Smad3 in the Wenyang Huoxue group was significantly lower than that in the model group (P < 0.01). The expression of Smad7 in the Wenyang Huoxue group was significantly larger than that in the model group (P < 0.01) and similar to that in the fosinopril group. It is conjectured that Wenyang Huoxue recipe may inhibit the expression of transforming growth factor β1 and Smad3, increase Smad7 expression, thereby affecting the transforming growth factor β/Smad signaling pathway, and thus play a role in the prevention and cure of renal interstitial fibrosis.

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