中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (11): 1985-1988.doi: 10.3969/j.issn.1673-8225.2012.11.020

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

心肌梗死模型大鼠心肌组织中白细胞介素17的表达**☆◆

陈昭喆1, 2,陈志坚1,曾秋棠1,刘学刚1,张  青1,刘立新1,朱云涛1,钱  程1,杜以梅1,赵  宁1   

  1. 1华中科技大学协和医院心内科,华中科技大学同济医学院心血管病研究所,湖北省生物靶向治疗研究重点实验室,湖北省武汉市   430022;2河南省信阳市中心医院心内科,河南省信阳市  464000
  • 收稿日期:2011-09-06 修回日期:2011-12-03 出版日期:2012-03-11
  • 通讯作者: 陈志坚,博士,主任医师,教授,华中科技大学协和医院心内科,湖北省武汉市 430022 drchenok@ sohu.com
  • 作者简介:陈昭喆☆,男,1970年生,湖北省孝感市人,汉族,华中科技大学同济医学院在读博士,副主任医师,主要从事冠心病防治及心血管介入治疗方面的研究。 sunfly1020@yahoo.com.cn
  • 基金资助:

    国家自然科学基金项目。课题名称:TNF-α诱导急性缺血性室颤启动基质的作用及分子机制(30770880);TNF-α诱导Cx43异常分布在心梗后室性心律失常发生中的作用(30971242)。

Expression of interleukin-17 in the myocardium of rats with myocardial infarction

Chen Zhao-zhe1, 2, Chen Zhi-jian1, Zeng Qiu-tang1, Liu Xue-gang1, Zhang Qing1, Liu Li-xin1, Zhu Yun-tao1, Qian Cheng1, Du Yi-mei1, Zhao Ning1   

  1. 1Department of Cardiology, Union Hospital, Huazhong University of Science and Technology, Wuhan  430022, Hubei Province, China; 2Department of Cardiology, Central Hospital of Xinyang, Xinyang  464000, Henan Province, China
  • Received:2011-09-06 Revised:2011-12-03 Online:2012-03-11
  • Contact: author: Chen Zhi-jian, Doctor, Chief physician, Professor, Department of Cardiology, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China drchenok@sohu. com
  • About author:Chen Zhao-zhe☆, Studying for doctorate, Associate chief physician, Department of Cardiology, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China; Department of Cardiology, Central Hospital of Xinyang, Xinyang 464000, Henan Province, China sunfly1020@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30770880*, 3097124*

摘要:

背景:白细胞介素17在多种心血管疾病中如自身免疫性心肌炎、病毒性心肌炎、扩张型心肌病、不稳定型冠心病等表达增加,在心血管病慢性炎症性过程起到了一定作用。
目的:分析白细胞介素17在心肌梗死大鼠心肌中的表达特点。
方法: 采用液氮冷冻法建立大鼠心肌梗死模型,将造模成功的66只大鼠按照不同的观察时间随机摸球法均分为1,3 ,7,14,28,56 d组,另设对照组8只。采用酶联免疫吸附测定法、免疫荧光激光共聚焦技术及蛋白免疫印迹法检测白细胞介素17的表达。
结果与结论:造模成功率为73%。大鼠心肌梗死的发生发展过程中,白细胞介素17在心肌组织大量表达,且随着时间变化而逐渐升高,在28 d时表达最为显著。说明白细胞介素17作为一种强力的促炎症因子在心肌梗死大鼠心肌组织中大量表达,有可能与心肌梗死后的心室重塑及心律失常的发生有关。
关键词:白细胞介素17;心肌梗死;大鼠;模型;心肌组织
doi:10.3969/j.issn.1673-8225.2012.11.020

关键词: 白细胞介素17, 心肌梗死, 大鼠, 模型, 心肌组织

Abstract:

BACKGROUND: Expression of interleukin-17 increases in many cardiovascular disease such as autoimmune myocarditis, viral myocarditis, dilated cardiomyopathy and unstable coronary artery disease. Interleukin-17 plays an important role in chronic inflammatory process of cardiovascular diseases.
OBJECTIVE: To study the expression of interleukin-17 in the myocardium after myocardial infarction in rats.
METHODS: Liquid nitrogen freezing method was used to establish a rat myocardial infarction model. Totally 66 rats with modeling successful were randomly divided into six groups according to different observation times: 1, 3, 7, 14, 28, 56 days groups. And eight rats were selected as controls. The expression of interleukin-17 was examined by enzyme linked immunosorbent assay, laser confocal scanning microscope and Western blot.
RESULTS AND CONCLUSION: Modeling success rate was 73%. With the development of myocardial infarction, interleukin-17 was abundantly expressed in the myocardial tissue and gradually increased over time. The expression of interleukin-17 in the 28 days group increased most significantly. Interleukin-17 as a potent pro-inflammatory factor can strongly express in rat myocardium after myocardial infarction, which may be related to the incidence of ventricular remodeling and arrhythmia.

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