中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (11): 1999-2002.doi: 10.3969/j.issn.1673-8225.2012.11.023

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

VX2肿瘤种植于肾静脉上下建立的兔下腔静脉模型*★

张元国,任  为   

  1. 重庆医科大学附属第一医院血管外科,重庆市  400016
  • 收稿日期:2011-11-19 修回日期:2011-12-31 出版日期:2012-03-11
  • 通讯作者: 任为,博士,副教授,重庆医科大学附属第一医院血管外科,重庆市 400016 renwei9771@yahoo.com.cn
  • 作者简介:张元国★,男,1987年生,山东省菏泽市人,汉族,重庆医科大学在读硕士,主要从事血管外科基础与临床方面研究。 cyzyg2009@163.com
  • 基金资助:

    重庆市卫生局医学科研项目面上项目(2010-2-041)。

Establishment of an inferior vene cava animal model by VX2 tumor implanted into the suprarenal and infrarenal vein in rabbits

Zhang Yuan-guo, Ren Wei   

  1. Department of Vascular Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China
  • Received:2011-11-19 Revised:2011-12-31 Online:2012-03-11
  • Contact: author: Ren Wei, Doctor, Associate professor, Department of Vascular Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China renwei9771@yahoo.com.cn
  • About author:Zhang Yuan-guo★, Studying for master’s degree, Department of Vascular Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China cyzyg2009@163.com
  • Supported by:

    Surface Projects of Medical Research Foundation of Chongqing Municipal Health Bureau, No.2010-2-041*

摘要:

背景:目前,临床上对恶性肿瘤侵犯肾静脉以上、以下的下腔静脉处理原则及方式还有争议,而类似的动物模型尚少见文献报道。
目的:分别建立VX2肿瘤侵犯肾静脉以上、以下的下腔静脉的动物模型,观察并对比分析其生长,以及对下腔静脉和腹主动脉的影响。
方法:将24只实验兔随机摸球法均分为肾静脉以上组和肾静脉以下组,分别将VX2肿瘤组织块种植在兔肾静脉以上、以下的下腔静脉附近软组织内,每周在超声下观察肿瘤的大小,下腔静脉和腹主动脉管径大小,对周围组织的侵犯情况。
结果与结论:所有肿瘤均种植成功。两组肿瘤大小与生长时间均呈显著正相关(r1=0.894,r2=0.879),下腔静脉管径及腹主动脉管径与肿瘤大小均显著负相关(r3=-0.663,r4=-0.834;r5=-0.826,r6=-0.870)。两组肿瘤大小变化差异无显著性意义    (P=0.293> 0.05)。在观察期末,肾静脉以上组下腔静脉管狭窄不到50%,肾静脉以下组下腔静脉管已完全闭塞,且有效避免了对腹主动脉的压迫(狭窄不到50%),结果说明VX2肿瘤种植于肾静脉以下可以得到较理想的动物模型。
关键词:下腔静脉;VX2瘤;兔;肿瘤大小;动物模型
doi:10.3969/j.issn.1673-8225.2012.11.023

关键词: 下腔静脉, VX2瘤, 兔, 肿瘤大小, 动物模型

Abstract:

BACKGROUND: At present, the principle and procedure for the treatment of tumor invading suprarenal and infrarenal inferior vene cave (IVC) are in dispute, but corresponding animal model is rarely reported in the literatures.
OBJECTIVE: To establish the experimental animal model of the VX2 tumor invading suprarenal and infrarenal IVC, and to observe the influence on tumor growth and the IVC and abdominal aorta (AA).
METHODS: Twenty-four rabbits were randomly divided into suprarenal group and infrarenal group, the prepared VX2 tumor tissue mass was transplanted into the soft tissues around the suprarenal and infrarenal IVC. Tumor size, diameter of the IVC and the AA, and the surrounding tissues were detected by ultrasound once a week as long as 5 weeks after implanted.
RESULTS AND CONCLUSION: All VX2 tumors had been growing successfully in the experimental rabbits. Tumor size was significantly positive related to the growth time in two groups (r1=0.894, r2=0.879); the diameter of the IVC and AA were significantly negative related to tumor size in two groups (r3=-0.663, r4=-0.834; r5=-0.826, r6=-0.870). The difference of tumor size was not significant between two groups (P1=0.293>0.05). In the observation period, the stenosis of IVC was less than 50% in suprarenal group; the IVC in infrarenal group was completely blocked and effectively avoided the pressure on the AA. VX2 tumor tissue mass transplanted into the soft tissues around the infrarenal IVC leads to an ideal animal model.
 

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