中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (11): 2023-2027.doi: 10.3969/j.issn.1673-8225.2012.11.029

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

不同给氢方式对兔氢气代谢的影响*☆

黄国庆,詹  蔚,熊  艳,文明祥,李颖庆,廖晓星   

  1. 中山大学附属第一医院急诊科,广东省广州市    510080
  • 收稿日期:2011-12-13 修回日期:2012-01-10 出版日期:2012-03-11
  • 通讯作者: 廖晓星,博士,博士生导师,教授,中山大学附属第一医院急诊科,广东省广州市 510080 liaowens@163.com
  • 作者简介:黄国庆☆,男,1979年生,福建省连江县人,汉族,中山大学附属第一医院急诊科在读博士,主要从事心肺脑复苏研究。 hgq97@126.com
  • 基金资助:

    中山大学青年教师基金(80000-3171914)。

Hydrogen metabolism in rabbits by different ways of hydrogen supply 

Huang Guo-qing, Zhan Wei, Xiong Yan, Wen Ming-xiang, Li Ying-qing, Liao Xiao-xing   

  1. Department of Emergency, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Received:2011-12-13 Revised:2012-01-10 Online:2012-03-11
  • Contact: author: Liao Xiao-xing, Doctor, Doctoral supervisor, Professor, Department of Emergency, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China liaowens@163.com
  • About author:Huang Guo-qing☆, Studying for doctorate, Department of Emergency, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China hgq97@126.com
  • Supported by:

    the Young Teachers Foundation of Sun Yat-sen University, No. 80000-3171914*

摘要:

背景:不同给氢方式均可对缺血再灌注损伤产生治疗作用,目前尚缺乏不同给氢方式对机体氢气代谢影响的研究。
目的:比较等体积氢水腹腔、静脉注射及氢气腹腔注射对兔氢气代谢的影响,以利于提供更优化的氢气治疗方案。
方法:20只新西兰大白兔在监测呼气氢气基础值后,依次予以饱和氢气生理盐水、氢气10 mL/kg腹腔注射及氢水10 mL/kg 10 min内静脉泵入,持续监测呼气氢气浓度,等降至基础值并稳定30 min以上后再进行下一方式给氢干预。记录各种给氢方式呼气氢气浓度的达峰时间、峰浓度,计算半衰期和曲线下面积,比较3种给氢方式对呼气氢气浓度的影响。
结果与结论:腹腔注射氢水、氢气及静脉注射氢水3种方式达峰时间分别为(3.25±1.80),(17.8±6.48)和(7.45±1.39) min,差异有显著性意义(P < 0.01);半衰期分别为(7.40±2.09),(141.50±85.01)和(1.20±0.37) min,差异有显著性意义(P < 0.01);较基础值增加的曲线下面积分别为(302.17±221.90),(5 234.29±2 681.18)和(209.51±104.49) ppm•min,差异具有显著性意义(P < 0.01)。提示腹腔注射氢水、氢气均可迅速提高呼气氢气浓度,腹腔注射氢气能长时间维持呼气氢气高浓度。
关键词:氢气;饱和氢气生理盐水;代谢;腹腔注射;静脉注射
doi:10.3969/j.issn.1673-8225.2012.11.029

关键词: 氢气, 饱和氢气生理盐水, 代谢, 腹腔注射, 静脉注射

Abstract:

BACKGROUND: Different ways to supply hydrogen have curative effect on ischemia-reperfusion injury. But the relevant metabolism studies are rare to compare the different ways of supplying hydrogen.
OBJECTIVE: To investigate the effect of intraperitoneal or intravenous saturated hydrogen saline and intraperitoneal injection of hydrogen on hydrogen metabolism in rabbits, providing a more optimal hydrogen treatment proposal.
METHODS: Twenty New Zealand white rabbits were tested the basis concentration of exhaled hydrogen. Then all of them were supplied saturated hydrogen saline or hydrogen gas 10 mL/kg via intraperitoneal injection and saturated hydrogen saline       10 mL/kg via intravenous injection within 10 minutes, respectively. Exhaled hydrogen concentrations were monitored continuously. When the exhaled concentration declined to the baseline and stabilized for more than 30 minutes, hydrogen was supplied by another way. Time to maximal exhaled hydrogen concentration and max exhaled hydrogen concentration were recorded, as well as half-life and area under the curve were calculated to compare the effects of three different ways of hydrogen supply on hydrogen metabolism in rabbits.
RESULTS AND CONCLUSION: The time to maximal exhaled hydrogen concentration was (3.25±1.80), (17.8±6.48) and (7.45±1.39) minutes in three ways, respectively, and there were significant differences among the three ways (P < 0.01). The half-life was (7.40±2.09), (141.50±85.01) and (1.20±0.37) minutes, respectively, and there were significant differences among the three ways (P < 0.01). The increment in the area under the curve was (302.17±221.90), (5 234.29±2 681.18), (209.51±     104.49) ppm•min, respectively, and there were significant differences among the three ways (P < 0.01). Hydrogen supply by intraperitoneal injection of saturated hydrogen saline or pure hydrogen both can rapidly increase exhaled hydrogen concentration in rabbits. Intraperitoneal hydrogen can maintain a high exhaled hydrogen concentration for a long time.

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