中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (11): 2028-2032.doi: 10.3969/j.issn.1673-8225.2012.11.030

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

微透析和高效液相色谱检测万古霉素在清醒兔眼玻璃体内的浓度★

王亚玲1,王洪格1,陈晓隆2   

  1. 1青岛市市立医院眼科,山东省青岛市  266001;2中国医科大学附属盛京医院眼科,辽宁省沈阳市  110006
  • 收稿日期:2011-02-23 修回日期:2011-09-29 出版日期:2012-03-11
  • 作者简介:王亚玲★,女,1968年生,山东省潍坊市人,汉族,2004年中国医科大学毕业,硕士,主要从事微透析技术在眼科中的应用方面的研究。

Microdialysis and high performance liquid chromatography detection for vancomycin concentration in vitreous chamber of conscious rabbits★

Wang Ya-ling1, Wang Hong-ge1, Chen Xiao-long2   

  1. 1Department of Ophthalmology, Qingdao Municipal Hospital, Qingdao  266001, Shandong Province, China; 2Department of Ophthalmology, Shengjing Hospital, China Medical University, Shenyang  110006, Liaoning Province, China
  • Received:2011-02-23 Revised:2011-09-29 Online:2012-03-11
  • About author:Wang Ya-ling★, Master, Department of Ophthalmology, Qingdao Municipal Hospital, Qingdao 266001, Shandong Province, China

摘要:

背景:万古霉素玻璃体内注射后的药代动力学资料较少。
目的:观察万古霉素玻璃体腔内注射后在清醒兔眼玻璃体内的浓度。
方法:将微透析探针植入清醒正常兔眼和细菌性眼内炎兔眼玻璃体内24 h后,向玻璃体内注射10 g/L万古霉素0.1 mL,利用微透析采样技术联合高效液相色谱法连续检测万古霉素注射后0.5,1,2,4,6,12,24,48,72,84 h,兔眼玻璃体内的药物浓度。
结果与结论:万古霉素在正常兔眼玻璃体内的代谢呈开放式二室模型,玻璃体内的峰值浓度为695.92 mg/L,半衰期51.66 h;在细菌性眼内炎兔眼玻璃体内的代谢呈一室模型,万古霉素的峰值浓度为713.35 mg/L,半衰期为11.91 h。所有动物给药84 h,玻璃体内万古霉素的浓度均高于最小抑菌浓度。实验在动物清醒状态下实时、连续、动态采样,检测结果准确,能满足万古霉素药动学分析的需要。

关键词: 微透析技术, 高效液相色谱法, 玻璃体, 万古霉素, 药代动力学

Abstract:

BACKGROUND: There are currently few studies regarding the pharmacokinetics of vancomycin via intravitreous injection.
OBJECTIVE: To determine the concentration of vancomycin injected into the vitreous chamber of conscious rabbits. 
METHODS: A microdialysis probe was implanted into vitreous chamber of normal rabbit eyes and rabbit eyes infected with bacterial endophthalmitis for 24 hours, and 10 g/L vancomycin 0.1 mL was administered intravitreally. The drug concentration in the vitreous chamber of rabbit eyes was determined at 0.5, 1, 2, 4, 6, 12, 24, 48, 72 and 84 hours after injection, through the microdialysis and high performance liquid chromatogram-ultraviolet detection.
RESULTS AND CONCLUSION: The metabolism of vancomycin showed an open two-compartment model in normal rabbit eyes. Its half-life was 51.66 hours and the peak concentration was 695.92 mg/L. The metabolism of vancomycin in the infected vitreous chamber showed a one-compartment model. Its half-life was 11.91 hours and the peak concentration was 713.35 mg/L. All rabbits were injected with drugs for 84 hours and the intravitreous concentration of vancomycin was higher than minimal inhibitory concentration. The experimental findings indicate that microdialysis coupled to high performance liquid chromatography is a powerful tool to investigate the ocular pharmacokinetics of vancomycin, and the samples are harvested in a real-time, continuous and dynamic fashion when the experimental animals are conscious.

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