中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (4): 597-605.doi: 10.3969/j.issn.2095-4344.1042

• 骨与关节综述 bone and joint review • 上一篇    下一篇

骨组织再生工程中富血小板血浆的应用与研究进展

汤其元1,马亚萍2,3,张 斌4,罗南宁1,王 信2,3,张 怡5   

  1. 1遵义医学院第一临床学院,贵州省遵义市 563000;2遵义医学院附属医院骨科,贵州省遵义市 563000;3遵义医学院-罗切斯特大学骨科研究中心,贵州省遵义市 563000;4贵州省骨科医院骨科,贵州省贵阳市  5500025遵义医学院公共卫生学院,贵州省遵义市 563000
  • 出版日期:2019-02-08 发布日期:2019-02-08
  • 通讯作者: 王信,博士,副主任医师,遵义医科大学附属医院骨科,遵义医科大学-罗切斯特大学骨科研究中心,贵州省遵义市 563000 张怡,博士,副教授,遵义医科大学公共卫生学院,贵州省遵义市 563000
  • 作者简介:汤其元,女,1994年生,贵州省人,汉族,遵义医科大学在读学士,主要从事骨缺损和骨愈合方面的研究。 共同第一作者:马亚萍,遵义医科大学附属医院骨科,遵义医科大学-罗切斯特大学骨科研究中心,贵州省遵义市 563000
  • 基金资助:

    国家自然科学基金(31760266),项目负责人:王信;遵义医学院大学生创新训练项目(遵医201751052),项目负责人:汤其元

Application and research progress of platelet-rich plasma in bone tissue regeneration  

Tang Qiyuan1, Ma Yaping2, 3, Zhang Bin4, Luo Nanning1, Wang Xin2, 3, Zhang Yi5   

  1. 1The First Clinical Institute, Zunyi Medical University, Zunyi 563000, Guizhou Province, China; 2Department of Orthopedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China; 3Joint Orthopedics Research Center of Zunyi Medical University & University of Rochester Medical Center, Zunyi 563000, Guizhou Province, China; 4Department of Orthopedics, Guizhou Provincial Orthopedics Hospital, Guiyang 550002, Guizhou Province, China; 5School of Public Health, Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • Online:2019-02-08 Published:2019-02-08
  • Contact: Wang Xin, PhD, Associate chief physician, Department of Orthopedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China; Joint Orthopedics Research Center of Zunyi Medical University & University of Rochester Medical Center, Zunyi 563000, Guizhou Province, China Zhang Yi, PhD, Associate professor, School of Public Health, Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • About author:Tang Qiyuan, The First Clinical Institute, Zunyi Medical University, Zunyi 563000, Guizhou Province, China Ma Yaping, Department of Orthopedics, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China; Joint Orthopedics Research Center of Zunyi Medical University & University of Rochester Medical Center, Zunyi 563000, Guizhou Province, China Tang Qiyuan and Ma Yaping contributed equally to this work.
  • Supported by:

     the National Natural Science Foundation of China, No. 31760266 (to WX); the College Students Innovation Training Program of Zunyi Medical University, No. 201751052 (to TQY)

摘要:

文章快速阅读:

 
 

 

文题释义:
富血小板血浆(PRP):自体有限体积的全血经离心后得到的血小板浓聚物。富血小板血浆中含有大量的细胞因子、生长因子及蛋白质。在组织再生过程中,血小板生长因子增强伤口愈合和再生。在口腔科、矫形外科等领域广泛运用于治疗骨缺损。
骨组织再生工程:是利用细胞生物学及工程学原理和技术,研究开发能修复和改善损伤骨组织形态和功能的生物替代物的一门学科。
 
摘要
背景:研究发现血小板浓缩物是一种自体全血离心后获得的富集大量血小板的血浆,具有促进组织愈合的能力,而富血小板血浆是其第1代血小板浓缩物,研究表明其具有诱导成骨效应。
目的:阐述富血小板血浆的制备、分类、血小板源生长因子的释放及其促进骨再生作用及临床应用,为进一步研究富血小板血浆在骨组织再生工程的作用及临床应用提供一定的理论基础。
方法:以“platelet-rich plasma,bone defect,bone regeneration,growth factors”及“富血小板血浆,骨缺损,骨再生,生长因子”为检索词,应用计算机在CNKI、PubMed数据库检索1970年7月至2018年7月相关文献共136篇。对富血小板血浆的制备、分类、血小板源生长因子的释放及其促进骨再生作用及临床应用进行综述。
结果与结论:富血小板血浆是通过控制离心来获得不同浓度血小板的血浆制品,其来源于外周血,具有来源丰富、取材方便、制备简单和可吸收的优点。富血小板血浆不仅具有止血的作用,而且含有多种高浓度的生长因子及纤维蛋白,为修复细胞和组织提供良好的生长环境和支架,还刺激软组织再生,促进伤口早期闭合,以及多种炎性因子调控损伤修复的环境,还可防止感染。富血小板血浆可加快修复损伤或骨缺损,联合其他疗法应用于再生医学中为帮助愈合、缩短愈合时间提供了新的思路,尤其是在骨、软骨和肌腱修复方面有着广泛的应用前景。

中国组织工程研究杂志出版内容重点:人工关节;骨植入物;脊柱骨折;内固定;数字化骨科;组织工程
ORCID: 0000-0001-8249-5446(汤其元)

关键词: 富血小板血浆, 骨缺损, 骨再生, 骨生长因子, 骨愈合

Abstract:

BACKGROUND: Platelet concentrates have been found to be a kind of platelet-rich plasma obtained by centrifugation of autologous whole blood, and has the ability to promote tissue healing. Platelet-rich plasma, as the first generation of platelet concentrates, can facilitate osteogenesis.

OBJECTIVE: To review the preparation, and classification of platelet-rich plasma, release of platelet-derived growth factor and its promoting function and clinical application of bone regeneration, so as to provide theoretical basis for further investigation on the effect and clinical application of platelet-rich plasma in bone regeneration.
METHODS: CNKI and PubMed databases were retrieved using the keywords of “platelet-rich plasma, bone defect, bone regeneration, growth factors” in English and Chinese, respectively for the articles published from July 1970 to July 2018. Totally 136 articles were searched. The preparation and classification of platelet-rich plasma, the release of platelet-derived growth factor and promote bone regeneration effect and clinical applications were summarized.
RESULTS AND CONCLUSION: Platelet-rich plasma is a plasma product with different concentrations of platelets obtained by controlling centrifugation. It is derived from peripheral blood and has the advantages of abundant source, convenient material, simple preparation and absorbability. Platelet-rich plasma not only has the effect of hemostasis, but contains various high concentrations of growth factors and fibrin, which provides a beneficial growth environment and scaffold for cells and tissues. Moreover, it can stimulate soft tissue regeneration, promote early closure of wound, and provides the environment for a variety of inflammatory factor repair and prevents infection. Platelet-rich plasma can accelerate the repair of damaged or bone defects. Combined with other therapies in regenerative medicine, it provides new ideas for promoting healing and shortening healing time, especially has a broad prospect in the repair of bone, cartilage and tendon. 

中国组织工程研究杂志出版内容重点:人工关节;骨植入物;脊柱骨折;内固定;数字化骨科;组织工程

Key words: Platelet-Rich Plasma, Bone and Bones, Bone Regeneration, Tissue Engineering

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