中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (31): 5041-5045.doi: 10.3969/j.issn.2095-4344.1334

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

整块石蜡包埋处理颈动脉粥样硬化斑块的方法

曹敬丽1,2,Marina S. Ferguson3,孙  杰3,张  东1,刘  丽1,李子瑞1,王雅杰1,隋滨滨1,沈  宓1,高培毅1,4,Thomas S. Hatsukami3,赵锡海5,苑  纯1,3,5   

  1.  (1首都医科大学附属北京天坛医院,国家神经系统疾病临床医学研究中心,北京市  100070;2脑血管病转化医学北京市重点实验室,北京市 100070;3美国华盛顿大学,血管成像实验室,美国,华盛顿-西雅图,98195;4北京市神经外科研究所,北京市  100070;5清华大学医学院,生物医学影像研究中心,北京市  100084)
  • 收稿日期:2019-05-09 出版日期:2019-11-08 发布日期:2019-11-08
  • 通讯作者: 苑纯,教授,博士生导师,首都医科大学附属北京天坛医院,国家神经系统疾病临床医学研究中心,北京市100070;美国华盛顿大学,血管成像实验室,美国,华盛顿-西雅图,98195;清华大学医学院,生物医学影像研究中心,北京市100084
  • 作者简介:曹敬丽,女,1979年生,山东省曲阜市人,汉族, 2014年毕业于北京协和医学院,博士,助理研究员,主要从事颈动脉粥样硬化组织学研究。
  • 基金资助:

    国家自然科学基金国际(地区)合作与交流项目(81361120402),项目负责人:高培毅

An en bloc paraffin embedding method for serial sectioning of carotid atherosclerotic plaque

Cao Jingli1, 2, Marina S. Ferguson3, Sun Jie3, Zhang Dong1, Liu Li1, Li Zirui1, Wang Yajie1, Sui Binbin1, Shen Mi1, Gao Peiyi1, 4, Thomas S. Hatsukami3, Zhao Xihai5, Yuan Chun1, 3, 5   

  1.  (1China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China; 2Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing 100070, China; 3Vascular Imaging Laboratory, Department of Radiology, University of Washington, Seattle 98195, WA, USA; 4Beijing Neurosurgical Institute, Beijing 100070, China; 5Center for Biomedical Imaging Research, Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China)
  • Received:2019-05-09 Online:2019-11-08 Published:2019-11-08
  • Contact: Yuan Chun, Professor, Doctoral supervisor, China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China; Vascular Imaging Laboratory, Department of Radiology, University of Washington, Seattle 98195, WA, USA; Center for Biomedical Imaging Research, Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China
  • About author:Cao Jingli, MD, Research assistant, China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China; Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing 100070, China
  • Supported by:

    Funding: the National Natural Science Foundation International (Regional) Cooperation and Exchange Project, No. 81361120402 (to GPY)

摘要:

文章快速阅读:

文题释义:
CEA斑块:是指经颈动脉内膜剥脱术取下的颈动脉粥样硬化斑块。
动脉粥样硬化的主要病理特征:为动脉某些部位的内膜下脂质沉积,并伴有平滑肌细胞和纤维基质成分的增殖,逐步发展形成动脉粥样硬化性斑块。
摘要
背景
:在颈动脉粥样硬化斑块的石蜡包埋工作中,将平均长度约3 cm的颈动脉粥样硬化斑块组织完整地包埋入蜡块中是上述科研工作必须步骤。
目的:阐述一种颈动脉粥样硬化斑块整块石蜡包埋处理方法。
方法:颈动脉粥样硬化斑块组织取自首都医科大学附属北京天坛医院经颈动脉内膜剥脱术手术取材样本。为了便于连续切片后的组织学层面能够与磁共振成像的信息作精准同层比对,实验在组织石蜡包埋时将颈动脉粥样硬化斑块组织作为一个整体完整地包埋入蜡块中。
结果与结论:在实际工作中,借鉴合作方美国华盛顿大学血管成像实验室在斑块处理方面的实践经验,结合实验室的实际情况,通过一种简易包埋方法进行颈动脉粥样硬化斑块的整块石蜡包埋,克服了颈动脉粥样硬化斑块组织整块包埋中的技术难点,为有类似工作需求的同行提供参考和引用。

关键词: 颈动脉粥样硬化斑块, 磁共振成像, 石蜡包埋, 包埋底模, 连续切片, 动脉粥样硬化, 责任斑块, 脑梗死, 稳定斑块, 不稳定斑块

Abstract:

BACKGROUND: Carotid atherosclerotic plaque with the length of about 3 cm completely embedding into paraffin block is a key step.
OBJECTIVE: To clarify a method to embed carotid atherosclerotic plaque en bloc in paraffin.
METHODS: Carotid atherosclerotic plaque was removed from the carotid endanerectomy at Beijing Tiantan Hospital, Capital Medical University. In order to facilitate the comparison between histological slices from serial sectioning and MRI, we need to embed carotid atherosclerotic plaque en bloc in paraffin.
RESULTS AND CONCLUSION: With the guidance of the University of Washington Vascular Imaging Lab, we had established a simple embedding method according to the practice in our lab. With the method, we successfully overcame the technical difficulties in the en bloc embedding of carotid atherosclerotic plaque, which provides a reference for researchers with similar work needs.

Key words: carotid atherosclerotic plaque, magnetic resonance imaging, paraffin embedding, embedding molds, serial section, atherosclerosis, culprit plaque, stroke, stable plaque, unstable plaque

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