中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (34): 5531-5537.doi: 10.3969/j.issn.2095-4344.1446

• 生物材料综述 biomaterial review • 上一篇    下一篇

自组装短肽水凝胶与骨组织构建

熊  娜,刘燕飞,卫  巍
  

  1. 遵义医科大学附属医院贵州省细胞工程重点实验室,贵州省遵义市  563003
  • 收稿日期:2019-05-30 出版日期:2019-12-08 发布日期:2019-12-08
  • 通讯作者: 刘燕飞,博士,副研究员,硕士生导师,遵义医科大学附属医院贵州省细胞工程重点实验室,贵州省遵义市 563003
  • 作者简介:熊娜,女,湖北省襄阳市人,汉族,遵义医科大学在读硕士,主要从事自组装短肽特性及组织工程方向的研究。
  • 基金资助:

    贵州省卫计委资助项目(Gzwjkj2017-1-036),项目负责人:刘燕飞;贵州省教育厅自然科学研究项目(黔教合KY字[2015]418),项目负责人:刘燕飞

Self-assembling peptide hydrogels and bone tissue construction

Xiong Na, Liu Yanfei, Wei Wei 
  

  1. Key Laboratory of Cell Engineering in Guizhou Province, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou Province, China
  • Received:2019-05-30 Online:2019-12-08 Published:2019-12-08
  • Contact: Liu Yanfei, PhD, Associate researcher, Master’s supervisor, Key Laboratory of Cell Engineering in Guizhou Province, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou Province, China
  • About author:Xiong Na, Master candidate, Key Laboratory of Cell Engineering in Guizhou Province, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou Province, China
  • Supported by:

    Project of the Health and Family Planning Commission of Guizhou Province, No. Gzwjkj2017-1-036 (to LYF); the Natural Science Research Project of Education Department of Guizhou Province, No. Qianjiaohe-KY[2015]418 (to LYF)

摘要:

文章快速阅读:

 

文题释义:
自组装短肽水凝胶:由相互交替的亲水性和疏水性氨基酸序列组成,在一定条件(离子浓度、温度、pH 值等)刺激下能够自发且有规律地通过非共价键作用形成稳定的β折叠结构,再进一步堆叠为纳米纤维,纳米纤维形成交织基质,最终形成含水量超过99%的水凝胶支架。
骨组织工程:由种子细胞、支架材料和细胞因子3个主要元素组成,是指将分离的自体高浓度成骨细胞、间充质干细胞或其他种子细胞,经体外培养扩增后种植于一种天然或人工合成的支架上,该支架具备良好的生物相容性和机械强度,可为细胞提供生存的三维空间,并且不诱发机体的不良反应;然后将包裹种子细胞的材料植入骨缺损部位,随着生物材料逐步降解,细胞不断增殖分化形成新组织,从而达到修复骨组织缺损的目的。
 
 
背景:与传统生物材料相比,自组装短肽水凝胶具有良好的生物相容性、低免疫原性、高含水量及降解产物可被机体重吸收利用等优点,在细胞三维培养、药物/蛋白缓释和组织工程等方面有广泛的应用。
目的:简述自组装短肽水凝胶在骨组织工程中的研究进展。
方法:应用计算机检索中国生物医学文献数据库、CNKI 数据库、PubMed 数据库及 Elsevier 数据库中2000至2019年发表的相关文献,中文检索关键词为“自组装短肽,水凝胶,骨缺损,成骨细胞,骨组织工程,生物材料”,英文检索关键词为“self-assembling peptides,hydrogels,bone defect,osteoblasts,bone tissue engineering,biomaterials”。
结果与结论:自组装短肽水凝胶的结构类似于天然细胞外基质,具有可编程、成本低、生物相容性良好、降解产物无毒性等优点,能够增强间充质干细胞和小鼠胚胎成骨细胞前体细胞MC3T3-E1及其他种子细胞的增殖和成骨分化,促进骨组织愈合与再生,可作为骨组织工程中的支架材料参与骨缺损的修复。但自组装短肽水凝胶低pH值有损伤细胞的可能,因此需设计出不同的生物活性序列对短肽进行改造,从而提高其生物活性、生物相容性和生物稳定性。

关键词: 自组装短肽, 水凝胶, 骨缺损, 成骨细胞, 干细胞, 骨组织工程, 生物材料, 生物相容性

Abstract:

BACKGROUND: Compared with traditional biomaterials, self-assembling peptide hydrogels have been widely used in three-dimensional cell culture, drug/protein release, tissue engineering, hemostasis and antibacterial agents because of its good biocompatibility, low immunogenicity, high water content, and the degradation product amino acids that can be absorbed and utilized by organism.
OBJECTIVE: To summarize the research progress of self-assembling peptide hydrogel in bone tissue engineering.
METHODS: A computer-based search of CBM, CNKI, PubMed and Elsevier was performed for retrieving articles concerning self-assembling peptide hydrogels in bone tissue engineering published from 2000 to 2019. The search terms were “self-assembling peptides, hydrogels, bone defect, osteoblasts, bone tissue engineering, biomaterials” in Chinese and English, respectively.
RESULTS AND CONCLUSION: Self-assembling peptide hydrogels have the structure similar to the natural extracellular matrix. The structure has the advantages of being programmable, low cost, good biocompatibility, and nontoxic degradation products. It can enhance the proliferation and osteogenic differentiation of mesenchymal stem cells and mouse embryonic osteoblast precursor cells (MC3T3-E1) as well as other seed cells and promote bone healing and regeneration. Therefore, self-assembling peptide hydrogels can be used as scaffold materials in bone tissue engineering to participate in the repair of bone defects. However, the low pH of self-assembling peptide hydrogels may damage cells, so different biologically active sequences should be designed to modify the peptide to enhance its biological activity, biocompatibility, and biostability.

Key words: self-assembling peptides, hydrogels, bone defect, osteoblasts, stem cells, bone tissue engineering, biomaterial, biocompatibility

中图分类号: