中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (4): 606-611.doi: 10.3969/j.issn.2095-4344.1939

• 生物材料综述 biomaterial review • 上一篇    下一篇

Zn、Mg增强羟基磷灰石骨修复材料临床应用与机制:生物活性及成骨诱导的研究进展

张亚楠1,2,严  霞1,2,孟增东2,3   

  1. 1昆明理工大学医学院,云南省昆明市  650500;2昆明理工大学附属医院骨科,云南省昆明市  650032;3云南省第一人民医院骨科,云南省昆明市  650032
  • 收稿日期:2019-04-16 修回日期:2019-04-20 接受日期:2019-06-12 出版日期:2020-02-08 发布日期:2020-01-07
  • 通讯作者: 孟增东,博士,主任医师,教授,昆明理工大学附属医院骨科,云南省昆明市 650032;云南省第一人民医院骨科,云南省昆明市 650032
  • 作者简介:张亚楠,女,1993年生,山西省忻州市人,汉族,昆明理工大学医学院、昆明理工大学附属医院在读硕士,主要从事生物骨缺损修复材料研究。
  • 基金资助:
    国家自然科学基金项目(31860264)

Zn and Mg increase the bioactivity and osteogenic induction of hydroxyapatite biomaterial in bone repair: clinical application and mechanism 

Zhang Yanan1,2, Yan Xia1,2, Meng Zengdong2, 3   

  1. 1Medical School of Kunming University of Science and Technology, Kunming 650500, Yunnan Province, China; 2Department of Orthopedics, the Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, China; 3Department of Orthopedics, the First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China
  • Received:2019-04-16 Revised:2019-04-20 Accepted:2019-06-12 Online:2020-02-08 Published:2020-01-07
  • Contact: Meng Zengdong, MD, Chief physician, Professor, Department of Orthopedics, the Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, China; Department of Orthopedics, the First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China
  • About author:Zhang Yanan, Master candidate, Medical School of Kunming University of Science and Technology, Kunming 650500, Yunnan Province, China; Department of Orthopedics, the Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 31860264

摘要:

文题释义:
骨缺损修复材料:目前临床上应用的骨修复材料主要有自体骨、同种异体骨和动物源性异种骨,但都存在一定的局限,应用受到限制。因此,研究开发对病损或缺失的骨组织进行有效修复和功能重建的人工骨修复材料具有广泛的临床需求和重要的意义,成为生物医用材料研究的热点。
骨组织工程:主要由3部分组成,分别是种子细胞、诱导因子及支架材料,通过培养的细胞在预制形态的三维支架上生长,之后植入骨缺损部位,在生物材料逐步降解的同时,种植的骨细胞不断增殖,从而达到修复骨组织缺损的目的。


背景:目前临床大量需求的自体骨存在来源不足、供骨区并发症等问题,而同种异体骨和异种骨存在免疫排斥、疾病传播等问题,应用受到限制,人工骨修复材料为骨缺损修复提供了重要解决途径。

目的:综合叙述锌、镁这2种二价阳离子掺杂羟基磷灰石的国内外最新基础研究进展及成骨诱导机制。

方法:检索PubMed数据库、万方数据知识服务平台及CNKI中国期刊全文数据库2006至2019年收录的有关Zn、Mg增强羟基磷灰石骨修复材料生物活性及成骨诱导相关研究的文章,检索词为“锌,镁,羟基磷灰石,体内,体外,成骨活性,骨修复,骨缺损;Zinc,magnesium, hydroxyapatite,in vivo,in vitro,osteogenic activity,bone repair,bone defect”。

结果与结论:尽管可降解活性元素Mg和Zn及其在羟基磷灰石基骨修复材料上的应用有较多研究,但研究目前仍然主要集中在金属或合金植入体方面,对于Mg-羟基磷灰石和Zn-羟基磷灰石复合材料的研究也大多制备成纳米颗粒、致密块体材料或作为生物医用金属材料的活性涂层来应用,关于将Mg和Zn元素与羟基磷灰石功能复合制备成仿骨结构多孔骨修复材料方面的研究还很少。同时金属离子材料仍面临许多挑战:首先,当特定金属从支架、植入物或其他释放装置中局部释放时,需要深入了解它们在健康和病变组织的细胞调控和细胞-细胞信号传导中的作用;其次,大量的体内实验证实金属离子可以从支架局部释放而不会产生全身毒性,但有致癌效应;此外还需要有更广泛的知识,了解将支架改善的生物学性能与金属离子释放效应联系起来的机制。


ORCID: 0000-0001-8645-7623(张亚楠)

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

关键词: 羟基磷灰石, 锌, 镁, 复合材料, 生物活性, 转录因子2, 成骨细胞, 破骨细胞

Abstract:

BACKGROUND: Autologous bones are in high demand in the clinic, but its clinical application is limited because of insufficient sources and donor-site complications. Allogeneic bone and xenogenic bone are also limited because of immune rejection and disease transmission problems. Artificial biomaterials provide an important solution to bone defect repair.

OBJECTIVE: To review the latest basic research progress and osteogenic induction mechanism of hydroxyapatite doped with divalent cations such as zinc and magnesium.

METHODS: A computer-based online search of Pebmed, Wanfang and CNKI databases was performed to retrieve papers regarding bone repair with hydroxyapatite doped with divalent cations zinc and magnesium with the search terms “Zinc, magnesium, hydroxyapatite, in vivo, in vitro, osteogenic activity” in English and Chinese respectively.

RESULTS AND CONCLUSION: Much is reported on the application of degradable active elements Mg and Zn and their application in hydroxyapatite for bone repair. However, most studies focus on metal or alloy implants. Mg-hydroxyapatite and Zn-hydroxyapatite composites are mostly prepared into nano-particles, dense bulk materials or active coatings of biomedical metal mateirals. Little is reported on bone-like porous bone repair materials involving Mg-hydroxyapatite and Zn-hydroxyapatite. At the same time, metal ion materials still face many challenges: First, when specific metals are released locally from stents, implants, or other release devices, their role in cell regulation and cell-cell signaling in healthy and diseased tissues should be known. Second, a large number of in vivoexperiments have confirmed that metal ions can be released from the stent without systemic toxicity, but have carcinogenic effects. Third, there is a need to further understand the mechanism linking biological property improvement and metal ion release. 

Key words: hydroxyapatite, zinc, magnesium, composite material, biological activity, transcription factor 2, osteoblast, osteoclasts

中图分类号: