中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (50): 8190-8194.doi: 10.3969/j.issn.2095-4344.2015.50.027

• 干细胞研究方案 stem cells study protocols • 上一篇    下一篇

人胎盘来源间充质干细胞对妊娠期高血压病细胞滋养细胞凋亡的影响:随机对照实验方案

张 莉1,李 治2,王 景2,刘 伟2   

  1. 1沈阳市妇婴医院,辽宁省沈阳市 110011;2沈阳医学院附属中心医院,辽宁省沈阳市 110024
  • 收稿日期:2015-10-12 出版日期:2015-12-03
  • 通讯作者: 刘伟,沈阳医学院附属中心医院,辽宁省沈阳市 110024
  • 作者简介:张莉,女,1980年生,辽宁省人,2011年中国医科大学毕业,博士,副主任医师,主要从事病理产科及生殖医学研究。

Effect of human placenta-derived mesenchymal stem cells on cytotrophoblast apoptosis in gestational hypertension: study protocol for a randomized controlled trial

Zhang Li1, Li Zhi2, Wang Jing2, Liu Wei2   

  1. 1Shenyang Women’s and Children’s Hospital, Shenyang 110011, Liaoning Province, China; 2Shenyang Central Hospital of Shenyang Medical University, Shenyang 110024, Liaoning Province, China
  • Received:2015-10-12 Online:2015-12-03
  • Contact: Liu Wei, Shenyang Central Hospital of Shenyang Medical University, Shenyang 110024, Liaoning Province, China
  • About author:Zhang Li, M.D., Associate chief physician, Shenyang Women’s and Children’s Hospital, Shenyang 110011, Liaoning Province, China

摘要:

背景:细胞滋养细胞凋亡增加、浸润能力下降是妊娠期高血压病发病的重要环节之一。人胎盘来源间充质干细胞已经在许多研究中显示出了对损伤的修复作用,而且胎盘间充质干细胞还具有一定的内分泌功能,可通过自分泌或旁分泌细胞因子的方式作用于近、远处的组织。因此,尝试移植人胎盘来源间充质干细胞修复损伤的滋养细胞功能,进而治疗妊娠期高血压病,有一定可行性。
方法/设计:随机对照细胞学实验。收集人胎盘来源间充质干细胞培养液,过滤后即为人胎盘来源间充质干细胞条件培养基。培养人绒毛膜滋养层细胞系JEG-3细胞,随机分为3组,对照组在培养液中加入体积分数15%正常孕妇血清;妊娠期高血压病模型组在培养液中加入体积分数15%子痫前期重度患者的血清;人胎盘来源间充质干细胞条件培养组在培养液中加入体积分数15%子痫前期重度患者血清培养24 h后,更换为体积分数15%子痫前期重度患者血清+人胎盘来源间充质干细胞条件培养基。采用流式细胞仪检测各组细胞的凋亡率。
讨论:实验从人胎盘来源间充质干细胞对妊娠期高血压病细胞滋养细胞功能的影响入手,可能为妊娠期高血压疾病的治疗找到一条细胞移植之路。
伦理批准:研究方案取得沈阳医学院附属中心医院临床研究伦理委员会的书面批准。正常及子痫前期孕妇签署知情同意书。
 

关键词: 干细胞, 移植, 胎盘间充质干细胞, 细胞滋养细胞, 妊娠期高血压, 细胞凋亡

Abstract:

BACKGROUND: Excessive apoptosis and decreased infiltration of cytotrophoblasts are essential causes for hypertension in pregnancy. Human placenta-derived mesenchymal stem cells contribute to damage repair, which has been shown in many studies, and moreover, human placenta-derived mesenchymal stem cells have a certain endocrine function and can act on the other tissues in an autocrine or paracrine manner. Therefore, we attempt to explore whether the human placenta-derived mesenchymal stem cell can repair damaged cytotrophoblasts, and then to gestational hypertension.
 
METHODS/DESIGN: This is a randomized controlled cytological experiment. Human placenta-derived mesenchymal stem cell culture medium is collected and filtrated as human placenta-derived mesenchymal stem cell conditioned medium. Human cytotrophoblasts, JEG-3 cells, are cultured and randomized into three groups:15% normal pregnant serum is added in control group; 15% serum from severe pre-eclampsia patients is added in gestational hypertension model group; and in condition medium group, 15% serum from severe pre-eclampsia patients is added for 24 hours of culture, and then human placenta-derived mesenchymal stem cell conditioned medium containing 15% serum from severe pre-eclampsia patients is used instead. Flow cytometry is used to detect cell apoptosis rate.
DISCUSSION: This study will help to find the feasibility of cell transplantation for gestational hypertension by exploring the effect of human placenta-derived mesenchymal stem cells on cytotrophoblasts function in gestational hypertension.
ETHICAL APPROVAL: The protocol is approved by the Ethics Committee of Shenyang Central Hospital of Shenyang Medical University. Informed consent is obtained from normal and pre-eclampsia women in pregnancy. 
 

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