中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (16): 2606-2611.doi: 10.3969/j.issn.2095-4344.2017.16.025

• 组织构建综述 tissue construction review • 上一篇    下一篇

组织工程窦房结构建及血管化的最新研究与应用进展

童灵犀1,张传森2,黄  飞1   

  1. 1滨州医学院烟台校区,山东省烟台市  264003;2解放军第二军医大学,上海市  200433
  • 修回日期:2017-01-21 出版日期:2017-06-08 发布日期:2017-06-08
  • 通讯作者: 张传森,教授,解放军第二军医大学,上海市 200433
  • 作者简介:童灵犀,男,1989年生,安徽省来安县人,汉族,滨州医学院在读硕士,主要从事心脏组织工程研究。
  • 基金资助:

    国家自然科学基金(81071603,30970737);上海市自然科学重点基金(09JC1417700)

Construction and vascularization of tissue-engineered sinus node in cardiac tissue engineering

Tong Ling-xi1, Zhang Chuan-sen2, Huang Fei1   

  1. 1Yantai Branch of Binzhou Medical University, Yantai 264003, Shandong Province, China; 2the Second Military Medical University, Shanghai 200000, China
  • Revised:2017-01-21 Online:2017-06-08 Published:2017-06-08
  • Contact: Zhang Chuan-sen, Professor, the Second Military Medical University, Shanghai 200000, China
  • About author:Tong Ling-xi, Studying for master’s degree, Yantai Branch of Binzhou Medical University, Yantai 264003, Shandong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81071603, 30970737; the Natural Science Foundation of Shanghai, No. 09JC1417700

摘要:

文章快速阅读:

文题释义:
组织工程窦房结:
利用支架材料与起搏细胞相结合所构建的具有一定起搏功能、能够代替病损窦房结的一类心脏组织工程。起搏细胞的选取种类多样大致为窦房结原代细胞,脂肪、髓间充质干细胞,胚胎干细胞等等,支架材料的选择也因种子细胞而异。                                     
组织工程的血管化:组织工程组织的存活需要血管提供氧气等营养物质,带走代谢产物,移植体内后的存活需要依赖宿主的血管长入,但大多数宿主血管都无法长入具有一定体积的移植体,血管化对于组织工程移植的早期成活非常重要。

 

摘要
背景:
理论上组织工程窦房结移植入体内可以建立新的起搏点,并且可以随着机体发育,达到治疗窦房结综合征的目的,但目前仍处于最初级的阶段,需要进行不断的探索。
目的:总结综述现阶段组织工程窦房结构建的不同方法及心脏组织工程血管化的研究进展。
方法:由第一作者检索PubMed 数据库及CNKI 全文数据库1984年至2016 年的相关文献,并进行筛选,归纳和总结。英文检索词为“Vascularize,Tissue Engineering,Sinus Node”,中文检索词为“血管化、组织工程、窦房结”。阐述组织工程窦房结的构建方法,总结归纳了各种心脏组织工程血管化的重要性及策略,分析组织工程窦房结血管化应用的方法,最后组织工程窦房结血管化进行总结和展望。
结果与结论:起搏种子细胞的选择大致为窦房结原代细胞,脂肪、骨髓间充质干细胞,胚胎干细胞等。组织工程支架材料更是种类繁多,活体的窦房结本身就处于胶原支架上,因此胶原类材料更为适用于组织工程窦房结。3D打印技术、细胞膜片技术使血管化组织工程窦房结的构建和移植成为可能。移植早期的血液供应,是窦房结移植是否成功的关键,组织工程窦房结血管化的相关文献在仍处于空白状态,内皮祖细胞作为成血管能力较强的细胞已经有了巨大的进展,但是仍有一些缺陷,还面临着重大的挑战。

 

 

ORCID: 0000-0002-3193-6480(童灵犀)

关键词: 组织构建, 组织工程, 器官移植, 生物起搏, 种子细胞, 3D打印, 细胞膜片技术, 内皮祖细胞, 缓释支架, 国家自然科学基金

Abstract:

BACKGROUND: As in theory tissue-engineered sinus node can establish a new pacemaker in vivo when implanted into the human body, it is a promising treatment for sick sinus syndrome. But this method is immature and needs to be explored in depth.
OBJECTIVE: To overview the different construction methods of tissue-engineered sinus node, and the research progress of vascularization in cardiac tissue engineering.
METHODS: A computer-based retrieval in PubMed and CNKI databases was performed by the first author to search related papers published from 1984 to 2016 using the keywords of “vascularize, tissue engineering, sinus node” in English and Chinese, respectively. We summarized the construction methods of tissue-engineered sinus node and importance of vascularization in cardiac tissue engineering.
RESULTS AND CONCLUSION: Sinus node cells, bone marrow mesenchymal stem cells, adipose-derived mesenchymal stem cells and embryonic stem cells can be used as seed cells. There are a variety of materials used for constructing tissue-engineered scaffolds, among which, collagen is the best choice. Three-dimensional printing technology and cell-sheet techbology make it possible to construct and transplant tissue-engineered sinus node. The early blood supply is the key for the successful sinus node transplantation. However, the vascularized tissue-engineered sinus node has not yet been reported. Endothelial progenitor cells can promote angiogenesis, but further explorations are warranted as there are some existing defects.

 

 

Key words: Sinoatrial Node, Neovascularization, Physiologic, Tissue Engineering

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