中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (11): 1674-1679.doi: 10.3969/j.issn.2095-4344.1108

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

力生长因子在兔膝关节软骨细胞增殖和迁移中的作用

赵  会,周君琳,杨铁军,李一汉,郭  蒙   

  1. (首都医科大学附属北京朝阳医院骨科,北京市  100020)
  • 收稿日期:2018-10-07 出版日期:2019-04-18 发布日期:2019-04-18
  • 通讯作者: 赵会,首都医科大学附属北京朝阳医院骨科,北京市 100020
  • 作者简介:赵会,男,1982年生,河南省新乡市人,汉族,2010年北京大学医学部毕业,博士,主治医师,主要从事创伤骨科以及足踝外科方面的研究。
  • 基金资助:

    国家自然科学基金项目(81202124),项目负责人:赵会;北京市医院管理局“青苗”计划 (QML20160302),项目负责人:赵会

Role of mechano growth factor in the proliferation and migration of rabbit knee articular chondrocytes  

Zhao Hui, Zhou Junlin, Yang Tiejun, Li Yihan, Guo Meng   

  1. (Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China)
  • Received:2018-10-07 Online:2019-04-18 Published:2019-04-18
  • Contact: Zhao Hui, Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
  • About author:Zhao Hui, MD, Attending physician, Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81202124 (to ZH); the “Young Talents” Program of Beijing Municipal Administration of Hospitals, No. QML20160302 (to ZH)

摘要:

文章快速阅读:

文题释义:
力生长因子:是由胰岛素样生长因子基因编码的,在组织或者细胞处于应激和损伤修复时,通过对mRNA进行剪接编码而翻译产生的选择性剪接变异体蛋白,力生长因子可以有效促进肌肉细胞增殖,诱导成肌细胞向肌管细胞分化,同样也可以促进成骨细胞和韧带细胞的增殖和诱导分化。
转化生长因子β1/Smad信号通路:转化生长因子β1信号通路参与生物体细胞的生长和分化过程,包括细胞生长、细胞分化、细胞凋亡、细胞动态平衡等其他细胞功能。转化生长因子β1信号通路过程是:相关信号因子与Ⅱ型受体结合,Ⅱ型受体招募并磷酸化Ⅰ型受体,Ⅰ型受体再磷酸化受体调控的SMAD蛋白,这些蛋白再与SMAD4结合。再磷酸化受体调控的SMAD蛋白/SMAD4复合体作为转录因子在细胞核内聚集,参与目标基因表达的调控。

摘要
背景
:力生长因子在多种组织中表达,能够促进多种组织细胞的增殖和迁移。
目的:观察力生长因子对原代培养兔软骨细胞增殖和迁移的影响,以及内在机制探讨。
方法:取北京市维通利华实验动物技术有限公司提供的新西兰大白兔的膝关节软骨,剪碎后,采用酶消化法原代分离培养软骨细胞,取处于对数生长期第3代细胞随机分5组,分别以0,1,2,4,8 μg/L力生长因子进行干预24,48,72 h。
结果和结论:不同质量浓度的力生长因子均能促进兔软骨细胞的增殖和迁移,且效应具有剂量依赖性,当质量浓度为4 μg/L时力生长因子促增殖和迁移的效应最大。且随着力生长因子质量浓度逐渐增加,可明显增强兔软骨细胞中转化生长因子β1的表达,刺激Smad2/3的磷酸化。提示力生长因子对原代培养兔软骨细胞具有促增殖和迁移的作用,且具有剂量依赖性,可能是通过激活转化生长因子β1/Smad信号通路得以实现的。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0002-2351-9150(赵会)

关键词: 软骨细胞, 力生长因子, 增殖, 迁移, 转化生长因子β1, 软骨细胞, 成骨细胞, 韧带细胞, 膝关节软骨

Abstract:

BACKGROUND: Mechano growth factor exists in a wide variety of tissues, and it can enhance the proliferation and migration of cells in different tissues.
OBJECTIVE: To investigate the effect of mechano growth factor on the proliferation and migration of primary rabbit knee articular chondrocytes, and to explore the underlying mechanism.
METHODS: Knee cartilage was shredded after being harvested from New Zealand white rabbits (provided by Beijing Vital River Laboratory Animal Technology Co., Ltd.), and then chondrocytes were isolated and cultured by enzyme digestion. Passage 3 cells in logarithmic growth phase were harvested and divided into five groups, and then intervened by 0, 1, 2, 4 and 8 μg/L mechano growth factor for 24, 48 and 72 hours, respectively.
RESULTS AND CONCLUSION: Mechano growth factor could promote the proliferation and migration of rabbit knee articular chondrocytes in a concentration-dependent manner, and mechano growth factor at 4 μg/L exhibited the strongest efficacy. Mechano growth factor could increase the expression of transforming growth factor β1 and phosphorylation of Smad2/3 with concentration increasing. In summary, mechano growth factor can promote the proliferation and migration of rabbit knee articular chondrocytes dose-dependently, which may be via the transforming growth factor β1/Smad signaling pathway.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Cytokines, Smad Proteins, Transforming Growth Factors, Tissue Engineering

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