中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 761-765.doi: 10.3969/j.issn.1673-8225.2012.05.001

• 肝移植 liver transplantation • 上一篇    下一篇

大鼠原位肝移植模型的建立*☆

时  军,吴勤荣,罗文峰,王永刚   

  1. 江西省人民医院器官移植科,江西省南昌市 330006
  • 收稿日期:2011-07-23 修回日期:2011-10-20 出版日期:2012-01-29
  • 作者简介:时军☆,男,1957年生,辽宁省沈阳市人,汉族,1997年同济医科大学毕业,博士,教授、硕士生导师,主要从事器官移植方面的研究。 shijun6207@163.com
  • 基金资助:

    江西省主要学科学术和技术带头人培养计划任务书(2008DD01100),课题名称:活体肝移植的基础和临床应用研究。

Establishing a rat model of orthotopic liver transplantation 

Shi Jun, Wu Qin-rong, Luo Wen-feng, Wang Yong-gang   

  1. Department of Organ Transplant, Jiangxi People’s Hospital, Nanchang  330006, Jiangxi Province, China
  • Received:2011-07-23 Revised:2011-10-20 Online:2012-01-29
  • About author:Shi Jun☆, Doctor, Professor, Master’s supervisor, Department of Organ Transplant, Jiangxi People’s Hospital, Nanchang 33 0006, Jiangxi Province, China shijun6207@163.com
  • Supported by:

     the Major Discipline Academic and Technology Leader Develop Project of Jiangxi Province, No. 2008DD01100*

摘要:

背景:“二袖套法”原位肝移植模型的建立缩短了无肝期的时间,并且显著提高了老鼠肝移植后的存活率。
目的:在“二袖套法”的基础上,结合一些相关文献,建立稳定大鼠原位肝移植模型。
方法:用改进的二袖套法对75对SD大鼠行原位肝移植,移植中门静脉、肝下下腔静脉用袖套法进行吻合,肝上下腔静脉用缝合法吻合,胆道采用支架法进行胆道重建。
结果与结论:受体大鼠移植后一般状况良好,50例正式实验移植后1 d存活率94%,1周存活率为90%。供肝热缺血时间均接近0,供体手术时间(34.44±3.25) min,受体手术时间(49.07±4.93) min,无肝期(17.26±2.51) min,下腔静脉平均阻断时间约为20 min。说明只有熟练地掌握手术技巧,细致耐心的操作,最大程度减少各种并发症的发生,才能获得稳定的原位肝移植模型。

关键词: 二袖套法, 大鼠, 肝移植模型, 细致耐心, 手术技巧

Abstract:

BACKGROUND: Establishing a model of orthotopic liver transplantation by two-cuff technique can decrease the time without the liver and significantly increase the survival rate of the rats subjected to liver transplantation.
OBJECTIVE: To establish the rats models of orthotopic liver transplantation based on two-cuff technique in combination with some related literatures.
METHODS: Totally 150 Sprague Dawley rats‎ were treated with liver transplantation by improved two-cuff method in the portal vein, hepatic inferior vena cava under the law in line with cuff, liver superior and inferior vena cava were sutured with suture line. Biliary tract reconstruction was completed in bile duct stent law.
RESULTS AND CONCLUSION: Receptors rats were generally in good condition after operation. 1-day survival rate was 94.0% and 1-week survival rate was 90.0% in 50 cases of the formal experiment. The time for donor operation and receptor operation was (34.44±3.25) minutes and (49.07±4.93) minutes, respectively, and anhepatic time was (17.26±2.51) minutes. The average blocking time of inferior vena cava was 20 minutes. To master the operation technique, operate patiently and carefully and reduce various complications can get a stable rat model of orthotopic liver transplantation.
 

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