中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 775-778.doi: 10.3969/j.issn.1673-8225.2012.05.004

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

不同途径移植羊膜上皮细胞在肝脏的定居**★○

张海燕1, 2,朴正福1, 2,丁淑芹1, 2,任  锋1, 2,加茂功3○,樱川宣男3○,李  宁1, 2   

  1. 1首都医科大学附属北京佑安医院,北京市 100069;2北京市肝病研究所,北京市 100069;3 北里大学医疗卫生学部再生医学讲座,日本神奈川县229-1131
  • 收稿日期:2011-07-14 修回日期:2011-09-18 出版日期:2012-01-29
  • 通讯作者: 李宁,主任医师,教授,博士生导师,首都医科大学附属北京佑安医院,北京市 100069
  • 作者简介:张海燕★,女,1980年生,河北省唐山市人,汉族,2004年中国协和医科大学毕业,硕士,主要从事肝病基础方面的研究。 cellzhy@ yahoo.com.cn 并列第一作者:朴正福☆,男,1965年生,吉林省吉林市人,汉族,2002年日本医科大学毕业,博士,主要从事肝病基础方面的研究。zfpiao88@yahoo.com.cn
  • 基金资助:

    北京市教育委员会科技发展计划面上项目 (KM200810025009) ;留学人员科技活动择优资助(20080015)。

Engraftment of human amniotic epithelial cells injected by different routes in the liver 

Zhang Hai-yan1, 2, Piao Zheng-fu1, 2, Ding Shu-qin2, Ren Feng1, 2, Isao Kamo3○, Norio Sakuragawa3○, Li Ning1, 2   

  1. 1Beijing You-an Hospital Affiliated to Capital Medical University, Beijing 100069, China;      2Beijing Institute of Liver Disease, Beijing 100069, China; 3Department of Regenerative Medicine, School of Allied Health Sciences, Kitasato University, Kanagawa, Japan
  • Received:2011-07-14 Revised:2011-09-18 Online:2012-01-29
  • Contact: Li Ning, Chief physician, Professor, Doctoral supervisor, Beijing You-an Hospital Affiliated to Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Disease, Beijing 100069, China
  • About author:Zhang Hai-yan★, Master, Beijing You-an Hospital Affiliated to Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Disease, Beijing 100069, China cellzhy@yahoo.com.cn Piao Zheng-fu★, Doctor, Beijing You-an Hospital Affiliated to Capital Medical University, Beijing 100069, China; Beijing Institute of Liver Disease, Beijing 100069, China zfpiao88@yahoo.com.cn
  • Supported by:

    Science and Technology Development (General Program) of Education Committee of Beijing, No. KM200810025009; Science and Technology Activities Foundation for Selected Returned Overseas Chinese Scholars, No. 20080015 

摘要:

背景:细胞移植对肝脏疾病具有一定的疗效,与肝脏移植相比有其自身的优点,但有诸多问题尚待解决。
目的:探讨不同途径移植的人羊膜上皮细胞在肝脏内定居情况。
方法:从剖腹产后的人胎盘羊膜中分离人羊膜上皮细胞,PKH26荧光标记后计数1×107细胞通过大鼠腹腔、门静脉、尾静脉及肝脏内直接注入方式移植入大鼠体内。
结果与结论:门静脉、尾静脉及肝脏直接注入途径移植的细胞均在肝脏内定居,但移植细胞数过量时造成局部肝组织的缺血坏死等不良反应。腹腔途径移植入体内的细胞未转移肝脏内。通过门静脉移植入体内的人羊膜上皮细胞在大鼠肝脏内维持存活至少16 d。移植细胞过量时,导致肝脏血管堵塞及坏死。证明人羊膜上皮细胞至少在大鼠肝脏中存活2周,提示低免疫原性的羊膜来源细胞可成为肝脏病治疗的候选细胞之一。

关键词: 羊膜上皮细胞, 细胞移植, 肝脏, 生物材料, 终末期肝病

Abstract:

BACKGROUND: Cell transplantation has a certain effect for liver disease, and it has its own advantages compared with the liver transplantation, but there are many issues to be resolved.
OBJECTIVE: To investigate the engraftment of human amniotic epithelial cells (hAECs) injected by different routes in liver of rats.
METHODS: hAECs were separated from placenta and labeled by PKH26 fluorochrome. hAECs (1×107 cells) were transplanted into SD rats through enterocoelia, portal vein, caudal vein or injected directly into the liver. Liver frozen sections were prepared after 3 days. The distribution of hAECs in liver was detected by converted fluorescence microscope. And survival condition of hAECs injected through portal vein in liver was observed by time dependent.
RESULTS AND CONCLUSION: hAECs were successfully transplanted into SD rat liver injected by portal vein, caudal vein and the route of liver injection, but hAECs injected intraperitoneally were not found in the liver. The hAECs that injected by portal vein could live for at least 16 days. Excessive cell injection causes the vascular thrombosis in liver and leading to necrosis. hAECs can live 16 days above without transplantation rejection. It indicates that amnion source cells may become one of the biomaterials for the treatment of liver diseases.

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