中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 818-822.doi: 10.3969/j.issn.1673-8225.2012.05.014

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

新型免疫状态定量监测皮肤移植模型小鼠的特异性*☆

潘明新,钟利民,汪  艳,高  毅,蒋泽生   

  1. 南方医科大学附属珠江医院肝胆二科,南方医科大学再生医学研究所,广东省广州市  510282
  • 收稿日期:2011-12-08 修回日期:2012-01-17 出版日期:2012-01-29
  • 通讯作者: 蒋泽生,博士,副主任医师,副教授,南方医科大学附属珠江医院肝胆二科,南方医科大学再生医学研究所,广东省广州市 510282 sjiang@hotmail.com 高毅,南方医科大学附属珠江医院肝胆二科,南方医科大学再生医学研究所,广东省广州市 510282
  • 作者简介:潘明新☆,博士,主任医师,教授,博士生导师,主要从事肝胆外科方面的研究。pmxwxy@sohu.com

Specificity of new quantitative monitoring of immune status in skin transplantation model rats

Pan Ming-xin, Zhong Li-min, Wang Yan, Gao Yi, Jiang Ze-sheng   

  1. Second Department of Hepatobiliary, Affiliated Zhujiang Hospital, Institute for Regenerative Medicine of Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2011-12-08 Revised:2012-01-17 Online:2012-01-29
  • Contact: Jiang Ze-sheng, Doctor, Associate chief physician, Associate professor, Second Department of Hepatobiliary, Affiliated Zhujiang Hospital, Institute for Regenerative Medicine of Southern Medical University, Guangzhou 510282, Guangdong Province, China sjiang@hotmail.com Gao Yi, Second Department of Hepatobiliary, Affiliated Zhujiang Hospital, Institute for Regenerative Medicine of Southern Medical University, Guangzhou 510282, Guangdong Province, China
  • About author:Pan Ming-xin☆, Doctor, Chief physician, Professor, Doctoral supervisor, Second Department of Hepatobiliary, Affiliated Zhujiang Hospital, Institute for Regenerative Medicine of Southern Medical University, Guangzhou 510282, Guangdong Province, China mxwxy@sohu.com
  • Supported by:

    the National Natural Science Foundation of China, No.30972825*

摘要:

背景:器官移植后如何监测受者的免疫状态,并预测排斥反应的发生,从而调整免疫抑制剂的用量是当前面临的重要问题。
目的:探索新型免疫状态定量监测方法在小鼠皮肤移植模型中是否有抗原特异性。
方法:建立 C57→BALB/c皮肤移植排斥模型,分别输注 C57/BALB/c 混合脾细胞悬液及第三者对照DBA/BALB/c混合脾细胞,在细胞输注后检测2种混合细胞悬液比例变化及供体细胞杀伤率,同时设置 BALB/c→BALB/c 同系皮肤移植对照组,免疫低下裸鼠对照组及免疫抑制剂对照组与之对比。
结果与结论:C57/BALB/c悬液-移植排斥模型组和C57/BALB/c悬液-移植排斥模型免疫抑制剂组小鼠对供体 C57 脾细胞有强烈的特异性杀伤作用,其中C57/BALB/c悬液-移植排斥模型免疫抑制剂组特异性杀伤作用稍弱,而输注第三者对照DBA/BALB/c混合脾细胞的小鼠细胞注射2~4 h内没有明显特异杀伤作用,但免疫低下的裸鼠始终未表现出特异性杀伤。说明实验建立的抗原特异性免疫状态检测方法能够快速地检测出皮肤移植受体的免疫状态。

关键词: 羧基荧光素乙酰乙酸琥珀酰亚胺酯, 流式细胞仪, 细胞毒性试验, 移植排斥, 细胞示踪, 免疫监测

Abstract:

BACKGROUND: How to monitor the recipient’s immune status, and predict the occurrence of rejection after organ transplantation, in order to adjust the dosage of immunosuppressive agents is the important issue faced currently.
OBJECTIVE: To investigate antigen-specificity of new quantitative monitoring of immune status in skin transplantation model rats
METHODS: The C57→BALB/c skin graft injection model was established, the models were injected with C57/BALB/c mixed splenocytes suspension and third party DBA/BALB/c mixed splenocytes suspension respectively. The proportion of mixed cell suspension and killing rate of donor cells were detected after cells injection. The homologous series skin transplantation control group was setted, and compared with immunocompromised nude mice control group and immunosuppressant control group.   
RESULTS AND CONCLUSION: The C57/BALB/c suspension in model group and C57/BALB/c in immunosuppressant group had a strong specific cytotoxicity on C57 splenocytes of the donors. The specific cytotoxicity of C57/BALB/c suspension in immunosuppressant group was weak, and there was no obvious specific cytotoxicity in the cells at 2-4 hours after third party DBA/BALB/c mixed splenocytes injection. However, the cells of immunocompromised nude mice had no specific cytotoxicity. The antigen-specific immune status detection method established in the experiment can detect the immune status of skin transplant recipients rapidly.

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