中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 843-846.doi: 10.3969/j.issn.1673-8225.2012.05.020

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

青蒿琥酯对迟发型超敏反应小鼠脾脏T淋巴细胞的免疫调节作用***☆◆

李  覃1, 2,陈  虹2, 3,白淑芳3,韦  娜3,张  实3   

  1. 武警医学院,1免疫学教研室,    2天津市职业与环境危害生物标志物重点实验室,  3生药学教研室,天津市 300162
  • 收稿日期:2011-07-13 修回日期:2011-09-22 出版日期:2012-01-29
  • 通讯作者: 陈虹,博士,教授,博士生导师,武警医学院,天津市职业与环境危害生物标志物重点实验室,生药学教研室,天津市 300162 chenhongtian06@yahoo.com.cn
  • 作者简介:李覃☆,女,1978年生,汉族,河南省汝州市人,2007年西安交通大学毕业,博士,讲师,主要从事免疫药理学及临床免疫学的研究。 Tanli20042001@yahoo.com.cn
  • 基金资助:

    中国博士后科学基金面上项目(20090461498),天津市应用基础与前沿技术研究计划(11JCYBJC14600),中国博士后科学基金特别资助项目(201104796)。

Immunomodulatory activity of artesunate to T lymphocytes from the spleens of delayed-type hypersensitivity mice 

Li Qin1,2, Chen Hong2, 3, Bai Shu-fang3, Wei Na3, Zhang Shi3   

  1. 1Department of Immunology, 2Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, 3Department of Pharmacognosy, Medical College of Chinese People’s Armed Police Force, Tianjin  300162, China
  • Received:2011-07-13 Revised:2011-09-22 Online:2012-01-29
  • Contact: Chen Hong, Doctor, Professor, Doctoral supervisor, Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China; Department of Pharmacognosy, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China chenhongtian06@yahoo.com.cn
  • About author:Li Qin☆, Doctor, Lecturer, Department of Immunology, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China; Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China Tanli20042001@yahoo.com.cn
  • Supported by:

    the Postdoctoral Science Foundation of China (General Program), No. 20090461498*; Applied Basis and Frontier Technique Research Plan of Tianjin, No. 11JCYBJC14600*; China Postdoctoral Science Foundation, No. 201104796*

摘要:

背景:青蒿琥酯是青蒿素低毒、高效的衍生物,具有免疫调节功能,但其具体机制仍需研究阐明。
目的:初步探讨青蒿琥酯对迟发型超敏反应小鼠脾脏T淋巴细胞的免疫调节作用。
方法:建立迟发型超敏反应小鼠模型,40只小鼠随机数字表法均分正常对照组、青蒿琥酯干预组、p-38MAPK抑制剂组、基质对照组进行实验观察。T淋巴细胞转化实验检测小鼠T淋巴细胞增殖水平;Western blot方法检测p38丝裂酶原激活蛋白激酶(p38MAPK)蛋白的活性表达。
结果与结论:局部给药后青蒿琥酯明显减轻迟发型超敏反应小鼠耳肿胀、降低脾指数、抑制刀豆蛋白A诱导的T淋巴细胞增殖;同时减弱p38 MAPK的磷酸化活性表达。提示青蒿琥酯可以有效抑制小鼠迟发型超敏反应,其作用途径可能与抑制p38 MAPK信号通路有关。

关键词: 青蒿琥酯, 免疫调节, 迟发型超敏反应, 脾脏, T淋巴细胞, p38 MAPK

Abstract:

BACKGROUND: Artesunate is a derivant of high efficiency and low toxicity from artemisinin, which has been shown to affect immune response. However, the underlying mechanism remains elusive.
OBJECTIVE: To investigate the immunoregulatory mechanism of artesunate to T lymphocytes from the spleens of delayed-type hypersensitivity mice.
METHODS: The effects of artesunate on the ear swelling and spleen index were measured in delayed-type hypersensitivity mice. The transformation of spleen lymphocytes was used to detect the proliferation of T cells. Western blot assay was applied to investigate the p38 mitogen-activated protein kinase (MAPK) activation. The proliferation of T lymphocytes was detected by T lymphocyte transformation test. MAPK activity was detected by western blot method.
RESULTS AND CONCLUSION: Topical application of artesunate could suppress the increase in ear thickness, spleen index and ConA-induced T lymphocytes proliferation significantly. Furthermore, artesunate was observed to decrease the phosphorylation of p38 MAPK. These demonstrated that artesunate had an anti-delayed-type hypersensitivity effect through modulating p38MAPK pathways, which might be beneficial for immune-related diseases.

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