中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 923-926.doi: 10.3969/j.issn.1673-8225.2012.05.038

• 器官移植基础实验 basic experiments of organ transplantation • 上一篇    下一篇

人废弃胚胎体外培养成囊胚的实验室结局

王  娟,吕玉珍,赵  芳   

  1. 河南省焦作市妇幼保健院生殖医学中心,河南省焦作市  454000
  • 收稿日期:2011-08-08 修回日期:2011-10-17 出版日期:2012-01-29
  • 作者简介:王娟,女,1973年生,河南省焦作市人,汉族,2004年郑州大学毕业,主管检验师,主要从事生殖医学研究。 jzbjywj@163.com

Laboratory outcomes of discarded embryos cultured into blastocysts in vitro

Wang Juan, Lü Yu-zhen, Zhao Fang   

  1. Center for Reproductive Medicine, Maternal and Child Health Hospital of Jiaozuo, Jiaozuo  454000, Henan Province, China
  • Received:2011-08-08 Revised:2011-10-17 Online:2012-01-29
  • About author:Wang Juan, Competent examiner, Center for Reproductive Medicine, Maternal and Child Health Hospital of Jiaozuo, Jiaozuo 454000, Henan Province, China jzbjywj@163.com

摘要:

背景:有报道称可以利用废弃胚胎建立人类胚胎干细胞系,对形态发育滞后的胚胎冻融后移植仍有再利用的医学价值。
目的:观察人废弃胚胎在体外继续培养形成囊胚的潜能。
方法:收集接受体外受精-胚胎移植或卵胞浆内单精子注射患者治疗后第3天废弃的胚胎404枚,进行囊胚序贯培养。
结果与结论:①废弃胚胎404枚,形成囊胚 65枚,总囊胚形成率为16.1%。②囊胚形成与患者的病因分类、年龄、受精方式无相关性(P > 0.05)。③胚胎来源于≥三原核(3PN)的(6~8)细胞组的囊胚形成率最高(P < 0.05)。卵裂球数随细胞数的增加,囊胚率也增高,但>8细胞囊胚率反而下降、与<3细胞组的结果相近。④Ⅰ,Ⅱ胚胎形成囊胚速度快,形成率显著高于Ⅲ,Ⅳ胚胎(P < 0.05)。⑤有囊胚形成患者的临床妊娠率显著高于无囊胚形成患者(P < 0.05)。提示囊胚形成与患者病因分类、年龄、受精方式无相关性。优质胚胎与(6~8)细胞的胚胎囊胚形成率高,多原核胚胎形成囊胚的价值应引起关注。废弃胚胎再利用能预测临床结局,为人类胚胎干细胞提供有利资源。
关键词:人废弃胚胎;胚胎培养;囊胚形成;结局分析;胚胎干细胞
doi:10.3969/j.issn.1673-8225.2012.05.038

关键词: 人废弃胚胎v胚胎培养, 囊胚形成, 结局分析, 胚胎干细胞

Abstract:

BACKGROUND: Some reports have showed that the discarded embryos can be used to establish the human embryonic stem cell lines, after freezing and thawing, the transplantation of embryos that hysteretic in morphological development still has medical value.
OBJECTIVE: To study the potential of discarded embryos cultured into blastocyst in vitro.
METHODS: Totally 404 discarded embryos were collected from the patients treated with in vitro fertilization and embryo transfer (IVF_ET) or intracytoplasmic sperm injection (ICSI), and then preformed with blastocyst sequential training.
RESULTS AND CONCLUSION: ①The 404 discarded embryos formed 65 blastocyst, the total blastocyst formation rate was 16.1%. ②There was no relation between the blastocyst formation and the patient's etiology, age, fertilization methods (P > 0.05). ③The blastocyst in embryos derived from the ≥ 3 pronuclear (PN) embryos (6-8) cell group had the highest formation rate    (P < 0.05). The number of blastomeres increased with the number of cells and the blastocyst rate was also increased, but, when the cell number was more than 8, the blastocyst rates was dropped, and similar to < 3 cell group. ④The Ⅰ and Ⅱ embryos with the a high blastocyst formation rate that was higher than Ⅲ and Ⅳ embryos (P < 0.05). ⑤The clinical pregnancy rate of the patients with blastocyst formation was higher than those without blastocyst formation (P < 0.05). The blastocyst formation has no relation with the etiology of patients, age and fertilization methods. The good quality embryos and (6-8) cell embryos has the highest blastocyst formation rate, and the blastocysts formation value of the pronuclear embryos should be caused for concern. The recycle of the discarded embryos can predict clinical outcomes and provide favorable land resources for human embryonic stem cells.

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