中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 931-934.doi: 10.3969/j.issn.1673-8225.2012.05.040

• 器官移植学术探讨 academic discussion of organ transplantation • 上一篇    下一篇

腹腔间隔室综合征动物模型的构建特点★

畅庚云1,梅铭惠2   

  1. 1桂林医学院,广西壮族自治区桂林市  541004;   2桂林医学院附属医院,广西壮族自治区桂林市 541001
  • 收稿日期:2011-10-22 修回日期:2011-12-09 出版日期:2012-01-29
  • 通讯作者: 梅铭惠,教授,硕士生导师,主任医师,桂林医学院附属医院肝胆外科,广西壮族自治区桂林市 541004 mhmei616@yahoo.com.cn
  • 作者简介:畅庚云★,男,1984年生,山西省太原市人,汉族,桂林医学院在读硕士,医师,主要从事腹腔间隔室综合症方面的研究。 cgyfs@163.com

Establishment of an abdominal compartment syndrome animal model

Chang Geng-yun1, Mei Ming-hui2   

  1. 1Guilin Medical College, Guilin  541001, Guangxi Zhuang Autonomous Region, China; 2Affiliated Hospital of Guilin Medical College, Guilin  541001, Guangxi Zhuang Autonomous Region, China
  • Received:2011-10-22 Revised:2011-12-09 Online:2012-01-29
  • Contact: Mei Ming-hui, Professor, Master’s supervisor, Chief physician, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China mhmei616@yahoo.com.cn
  • About author:Chang Geng-yun★, Studying for master’s degree, Physician, Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China cgyfs@163.com

摘要:

背景:腹腔间隔室综合征导致多器官功能损害的具体机制尚不十分清楚,为深入研究其发病机制及病理生理过程,需要合适的动物模型。
目的:就腹腔间隔室综合征动物模型的制备过程中可能遇到的相关问题进行讨论,包括制备模型的标准、方法、动物的选择及监测腹压的方式。
方法:由第一作者检索PubMed数据及CNKI数据库1990/2011有关腹腔间隔室综合征及腹腔高压及相关动物模型方面的文献。
结果与结论:一个成功的腹腔间隔室综合征动物模型最好能保持腹腔压力的稳定,并能持续一定的时间,同时尽可能少的影响实验结果。所以无论选择腹腔灌注气体还是灌注液体模型的测量腹腔内压并维持压力的稳定是关键。腹腔灌注液体模型无法动态监测腹压,且压力波动较大,与之相比腹腔灌注气体模型测压较为方便,若使用电子供气系统动态监测则更为理想。生理模型是前瞻性的实验,但现有的生理模型有待改进。

关键词: 腹腔间隔室综合征, 腹内高压, 动物模型, 腹腔间隙综合征, 腹腔筋膜室综合征, 腹腔高压 

Abstract:

BACKGROUND: The mechanism of organ functional lesion cause by abdominal compartment syndrome (ACS) is unclear. The appropriate animal models should be established for the in-depth study of the pathogenesis and pathophysiology.
OBJECTIVE: To investigate the related issues you might encounter during the process, including the model preparation standard, method, animal selection and monitoring of abdominal pressure.
METHODS: The PubMed database and CNKI database from 1990 to 2011 were used to search the articles on ACS, abdominal high pressure and related animal model.
RESULTS AND CONCLUSION: A successful animal model of ACS should have to maintain the stability of intra-abdominal pressure, and can persist for some time. Meanwhile it should impact on the experimental results as little as possible. Therefore, the key is the measurement of the intra-abdominal pressure in intraperitoneal infusion of liquid of gas model as well as the maintenance of a stable pressure. Dynamic monitor of the abdominal pressure can not be preformed on the intraperitoneal infusion of liquid model, and the pressure fluctuations is significant; To compare with intraperitoneal infusion of liquid model, it is convenient to measure the intra-abdominal pressure on intraperitoneal infusion of gas model, and the utilization of electronic supply system to dynamic monitor the intra-abdominal pressure is desirable. The establishment of physical model is forward-looking experiment, but the existing physical models are needed to be improved.

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