中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (34): 5509-5514.doi: 10.3969/j.issn.2095-4344.2014.34.017

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

磷酰胆碱表面改性增强聚四氟乙烯心脏瓣膜材料的生物相容性

张 本1,2,龚德军1,张锡武1,徐同毅1,韩 林1,唐 昊1,陆方林1,徐志云1   

  1. 1解放军第二军医大学长海医院胸心外科,上海市 200433;2解放军广州军区广州总医院心血管外科中心,广东省广州市 510010
  • 修回日期:2014-06-08 出版日期:2014-08-20
  • 通讯作者: 徐志云,教授,解放军第二军医大学长海医院胸心外科,上海市200433
  • 作者简介:张本,男,1982年生,河北省沧州市人,回族,解放军第二军医大学在读博士,主治医师,主要从事心血管外科研究。 并列第一作者:龚德军,男,1975年生,江苏省淮安市人,汉族,主管技师,主要从事心血管外科研究。
  • 基金资助:

    人民解放军“十二五”医学科研重点项目(BWS11C008)

Phosphorylcholine coating enhances biocompatibility of expanded polytetrafluoroethylene used in polymeric prosthetic heart valves

Zhang Ben1, 2, Gong De-jun1, Zhang Xi-wu1, Xu Tong-yi1, Han Lin1, Tang Hao1, Lu Fang-lin1, Xu Zhi-yun1   

  1. 1Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2Centre of Cardiovascular Surgery, Guangzhou General Hospital of Guangzhou Military Region, Guangzhou 510010, Guangdong Province, China
  • Revised:2014-06-08 Online:2014-08-20
  • Contact: Xu Zhi-yun, Professor, Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • About author:Zhang Ben, Studying for doctorate, Attending physician, Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; Centre of Cardiovascular Surgery, Guangzhou General Hospital of Guangzhou Military Region, Guangzhou 510010, Guangdong Province, China Gong De-jun, Technician in charge, Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China Zhang Ben and Gong De-jun contributed equally to this work.
  • Supported by:

    the Military Medical Research during the Twelfth Five-Year Plan, No. BWS11C008

摘要:

背景:前期研究发现将膨体聚四氟乙烯单瓣同时植入绵羊降主动脉和左肺动脉20周,无明显血栓、钙化或降解,验证了其优良的特性,但在左肺动脉处有明显的新生内膜增生。

目的:观察磷酰胆碱表面改性超微孔膨体聚四氟乙烯的生物相容性变化。
方法:以磷酰胆碱对膨体聚四氟乙烯进行表面改性,观察改性前后膨体聚四氟乙烯表面形状、接触角、屈服点拉伸应力及弹性模量、蛋白吸附能力的变化。①溶血实验:在稀释人血中分别加入改性膨体聚四氟乙烯浸提液、膨体聚四氟乙烯浸提液、生理盐水及蒸馏水。②血小板计数实验:在健康人全血中分别加入改性膨体聚四氟乙烯、膨体聚四氟乙烯、高密度聚乙烯及酵母聚糖A。③血小板激活实验:在健康人全血中分别加入改性膨体聚四氟乙烯、膨体聚四氟乙烯、γ-球蛋白及酵母聚糖A。

结果与结论:与改性前比较,改性后膨体聚四氟乙烯的微孔面积显著减小(P < 0.001),亲水性及抑制蛋白吸附能力显著增强(P < 0.001),生物力学性能及溶血性能无明显变化。体外血小板计数和血小板激活实验显示,改性后膨体聚四氟乙烯的抗血栓性能显著增强。说明磷酰胆碱表面改性可以增强超微孔膨体聚四氟乙烯的抗血栓性和抗蛋白吸附能力,改善其生物相容性。


中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

关键词: 生物材料, 材料相容性, 聚四氟乙烯, 表面改性, 磷酰胆碱, 高分子材料, 生物相容性

Abstract:

BACKGROUND: Our preliminary study found that the monocusp valves made of ultramicropore expanded polytetrafluoroethylene (ePTFE) revealed no significant thrombus, calcification, or degradation 20 weeks after implanted into the descending aorta and the left pulmonary artery in sheep, which verified the good property of ePTFE. However, the surface of ePTFE in the left pulmonary artery was covered with obvious neointima.

OBJECTIVE: To assess the biocompatibility of phosphorylcholine-coated ePTFE.
METHODS: ePTFE surface was modified by phosphorylcholine derivative. Then the changes of surface shape, tensile stress at yield and elasticity modulus, water contact angle, and protein absorption capacity of ePTFE after surface modification were observed. (1) Hemolytic test: the leaching solution of phosphorylcholine-coated ePTFE, leaching solution of uncoated ePTFE, normal saline, and distilled water were added to the diluted human blood, respectively. (2) Platelet count test: the phosphorylcholine-coated ePTFE, uncoated ePTFE, high density polyethylene, and Zymosan A were added to the whole blood samples from healthy volunteers, respectively. (3) Platelet activation test: the phosphorylcholine-coated ePTFE, uncoated ePTFE, γ-Globulins, and Zymosan A were added to the whole blood samples from healthy volunteers, respectively.

RESULTS AND CONCLUSION: The mean micropore diameter of ePTFE was significantly decreased after phosphorylcholine coating (P < 0.001). The hydrophilicity and the ability of suppressing protein adsorption were significantly strengthened after phosphorylcholine coating (P < 0.001). Phosphorylcholine coating did not influence ePTFE in biomechanical properties and hemolytic test. The platelet count test and platelet activation test demonstrated that phosphorylcholine coating significantly improved anti-thrombus function of ePTFE. So, phosphorylcholine coating can enhance anti-thrombus function, suppress protein adsorption, and improve biocompatibility of ePTFE.


中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

Key words: polytetrafluoroethylene, phosphorylcholine, materials testing

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