Effects of ginsenoside on brain-derived neurotrophic factor and tyrosine kinase B mRNA expression in the hippocampal formation of aged rats*☆
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Publisher：Quzwzb Publish Time：Thursday, November 20, 2008
Source：Neural Regen Res,2008,3(7),723-7 |
Hong Lai1, Wensu Liu2, Zhaosheng Li1, Haihua Zhao1, Yongli Lü1
1Department of Anatomy, College of Basic Medical Science, China Medical University, Shenyang 110001, Liaoning Province, China
2School of Geophysics and Information Technology, China University of Geosciences, Beijing 100083, China
Hong Lai☆, Doctor, Professor, Master’s tutor, Department of Anatomy, College of Basic Medical Science, China Medical University, Shenyang 110001, Liaoning Province, China
Supported by Liaoning Natural Science Foundation, No.20062088*
Lai H, Liu WS, Li ZS, Zhao HH, Lü YL.Effects of ginsenoside on brain-derived neurotrophic factor and tyrosine kinase B mRNA expression in the hippocampal formation of aged rats. Neural Regen Res 2008;3(7):723-7
| Abstract
BACKGROUND: There are a limited number of studies involving the effects of ginsenosides, the active component of ginseng, on expression of hippocampal TrkB mRNA in aged rats.
OBJECTIVE: To observe expression of brain-derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB) mRNA in the hippocampal formation of aged rats, as well as changes after ginsenoside administrated.
DESIGN, TIME AND SETTING: A randomized, controlled experiment was performed at the Department of Anatomy, College of Basic Medical Sciences, China Medical University in March 2005.
MATERIALS: A total of 39 female, Wistar rats were randomly divided into 3 groups (n = 13 each): young (3-5 months old), aged (27 months old), and ginsenoside group (received 25mg/kg/d ginsenoside in the drinking water between 17 and 27 months of age).
METHODS: Following anesthesia, the rats were exsanguinated and perfused transcardially with chilled, heparinized, 0.9% saline. The brains were removed and post-fixed in 40 g/L paraformaldehyde/phosphate buffer for 20 minutes, and further incubated in 30% sucrose/phosphate buffer overnight.
MAIN OUTCOME MEASURES: In situ hybridization, immunohistochemistry, and image analysis were used to investigate expression of BDNF and TrkB mRNA in the hippocampal formation.
RESULTS: The expression levels of BDNF in the hippocampal CA3 and CA1 of aged rats was significantly less than the young group (t = 2.879, 1.814, 1.984, P < 0.05). BDNF expression was significantly greater in the dentate gyrus of the ginsenoside group, compared with the aging group (t = 1.943, P < 0.01). The expression of TrkB mRNA in the hippocampal CA3, CA1, and dentate gyrus of aged rats was less than the young group (t = 3.540, 3.629, 17.905, P < 0.01). TrkB mRNA expression in the CA3 region and dentate gyrus of the ginsenoside group was significantly greater compared with the aging group (t = 1.293, 3.386, P < 0.05, 0.01).
CONCLUSION: BDNF and TrkB mRNA expression in the hippocampal formation were reduced in the aged group. However, ginsenosides can increase BDNF and TrkB mRNA expression in the hippocampal formation.
Key Words: ginsenosides; brain-derived neurotrophic factor; tyrosine kinase B; hippocampus
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