【Title】

“Immunohistochemical analysis of the expression of Hes1 in adult mouse brain”living pictures

【Source】

Journal of Clinical Rehabilitative Tissue Engineering Research  January 1, 2010  Vol.14 No.14，2612-2616

【Author】

Huan Lin-chun1, Yang Xin-yu1, Zhao Wang-miao1, Zhao Yan1, 2, Zhao Jie1, 3, Zhang Qi1, Yang Shu-yuan1

【Setting】

Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin Neurology Institute, Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin  300052

【Found】

the National Natural Science Foundation of China, No. 30973090*; Natural Science Foundation of Tianjin, No. 09JCZDJC17200*; Scientific Research Development Program of the Higher Education Institutions of Tianjin, No. 20070202*; Foundation of Health Bureau of Tianjin*

【Abstract】

BACKGROUND: In recent years, it has been shown in studies that regulation of the expression of Hes1 is very important to maintain neural stem cells and regulate its differentiation during the development of embryonic nervous system. Moreover, upregulation of Hes1 expression is required for quiescent adult fibroblasts resuming proliferation. This indicates that there is a close relationship between Hes1 and some potential stem cells in adult. Therefore, the study of Hes1 expression in neural stem cells and its relationship with adult neurogenesis is put on the agenda. However, the expression of Hes1 in adult brain is still not clear.

OBJECTIVE: To observe the expression of Hes1 in adult mouse brain and the cell types of Hes1 positive cells.

METHODS: Totally 12 adult male C57BL/6 mice were randomly evenly divided into two groups by using the random number table. For the first group, the mouse brain was removed directly and immunohistochemical staining was used to detect the expression of Hes1 in the adult mouse brain. For the second group, Brdu was injected intraperitoneally to every mouse with a dosage of 200 mg/kg, once a day, for 3 days. The mouse brain was removed at 4 days and then double staining was carried out to observe and analysis the cell types of Hes1 positive cells in hippocampus.

RESULTS AND CONCLUSION: Hes1 was expressed in all the observed anatomical site where neural cells exist, Brdu positive cells nearly all expressed Hes1, NeuN positive cells all expressed Hes1, whereas GFAP positive cells did not express Hes1 completely. Hes1 was expressed in all neurons and neural stem cells, and glial cells did not express Hes1..

【JPG pictures】

2 figures click

Figure 1  Expression of Hes1 in different parts of mouse brain
Figure 2  Double staining of Hes1 with Brdu, NeuN and GFAP respectively

【Full text】