【Title】

“Primary culture of spiral ganglion cells from cochlea of neonatal C57 mice”living pictures

【Source】

Journal of Clinical Rehabilitative Tissue Engineering Research  January 1, 2010  Vol.14 No.14，2617-2620

【Author】

Wang Ting-kuo, Sun Hong

【Setting】

Department of Otorhinolaryngology-Head ＆ Neck Surgery, Third Xiangya Hospital, Central South University, Changsha   410013, Hunan Province, China

【Found】

the National Natural Science Foundation of China, No. 30371531*，30772402*; the Natural Science Foundation of Hunan Province, No. 02JJY2050*; the Scientific Research Foundation of Health Department of Hunan Province, No. B2006-063*; the Key Program of Science and Technology Project of Department of Science and Technology of Hunan Province, No. 2007SK2001*

【Abstract】

BACKGROUND: At present, there are differences in reporting culture condition of cochleal spiral ganglion cells (SGCs) in circle of primary cell culture. Individual method has poor repeatability, and is not beneficial for practical application.

OBJECTIVE: To primary culture and evaluate SGCs of neonatal C57 mice.

METHODS: Tissues from cochlear modiolus were acquired from neonatal C57 mice by microanatomy. SGCs from cochlear modiolus were cultured by trypsin digestion, differential velocity adherent technique combined with chemicals. Cell growth was observed by inverted phase contrast microscope and hematoxylin and eosin staining. Immunocytochemistry was employed to classify cell types. 

RESULTS AND CONCLUSION: After purification, the cell body of SGCs from cochlear modiolus was ellipse or triangle, with slender processes in cytoplasm. Nuclei were positive for Nuen (stained brown). Cytoplasm and axon were positive for β3-Tubulin (stained brown). These indicated that mouse SGCs were successfully cultured.

【JPG pictures】

4 figures click

Figure 1  Morphology of spiral ganglion cells at 24 h after culture; white arrows show spiral ganglion cells；black arrow shows fibroblasts (×100)
Figure 2  Hematoxylin and eosin staining of spiral ganglion cells (×200)
Figure 3  Immunocytochemistry for Nuen in spiral ganglion cells, positive reaction with brown color in cell body (×100)
Figure 4  Immunocytochemistry for β3-Tubulin in spiral ganglion cells, positive reaction with brown color in cytoplasm, cell membrane and neurite (×200)

【Full text】