【Title】

“Superparamagnetic iron oxide and chlormethylbenzamido-1, 1'-dioctadecyl-3, 3, 3', 3'-tetramethylindocarbocyanine labeling and tracing bone marrow mesenchymal stem cells”living pictures

【Source】

Journal of Clinical Rehabilitative Tissue Engineering Research  January 1, 2010  Vol.14 No.14，2513-2517

【Author】

Dou Xing-kui1, Guo Tao2, Si Yong-yu1, Wan Lin-jun1, Huang Qing-qing1, Kang Bo2  

【Setting】

Department of Anesthesiology, Second Affiliated Hospital of Kunming Medical College, Kunming  650101,

【Found】

Specific Research Project of Yunnan Science and Technology Bureau, No. 2008CD007*

【Abstract】

BACKGROUND: The success of cell therapy will depend on the ability to monitor the fate of transplanted cells in vivo. Superparamagnetic iron oxide (SPIO) labeling is an ideal magnetic resonance contrast medium, and it offers the potential for non-invasive tracking of implanted cells. The chlormethylbenzamido-1,1' - dioctadecyl- 3, 3, 3',3' - tetramethylindocarbocyanine (CM-DiI) labeling does not have cytotoxicity, so it cannot influence cell growth.

OBJECTIVE: To investigate the effects of SPIO and CM-DiI labeling and tracking on bone marrow mesenchymal stem cells (BMSCs).

METHODS: Porcine BMSCs were isolated and cultured by the whole bone marrow method. BMSCs were labeled with SPIO containing 50 mg/L Ferrum and CM-DiI. The labeled BMSCs were transplanted into porcine myocardial infarction model via intracoronary infusion. The frozen sections of the cardiac tissues were obtained after 4 weeks.

RESULTS AND CONCLUSION: Efficiency of SPIO and CM-DiI labeling BMSCs was nearly 100%. The SPIO and CM-DiI labeled BMSCs could be found in the cardiac muscle tissues at 4 weeks after transplantation. SPIO and CM-DiI labeling BMSCs were efficiently tracked in vivo.

【JPG pictures】

7 figures click

Figure 1  Porcine bone marrow-derived mesenchymal stem cells following 48-h adherence (×400)
Figure 2  3rd generation of porcine bone marrow-derived mesenchymal stem cells (×200)
Figure 3  Flow cytometry analysis of surface antigen of 3rd generation of porcine bone marrow-derived mesenchymal stem cells
Figure 4  Superparamagnetic iron oxide-labeled mesenchymal stem cells appear blue after Prussian blue staining (×400)
Figure 5  Bone marrow mesenchymal stem cells labeled with CM-DiI under fluorescent microscope (×200)
Figure 6  Prussian blue staining shows labeled bone marrow mesenchymal stem cells paralleled in the same direction after 4 weeks of intracoronary injection (×100)
Figure 7  Frozen sections of the cardiac tissues display red-fluorescent cells under microscopy (×100)

【Full text】