【Title】

“Isolation of human umbilical cord mesenchymal stem cells and differentiation into adipocytes and osteblasts”living pictures

【Source】

Journal of Clinical Rehabilitative Tissue Engineering Research  January 1, 2010  Vol.14 No.14，2492-2496

【Author】

He Shao-qing1, 2, Luo Zhen-yu1, 2, Liu Qiu-ying1, 2, Zhou Xiang-rong2, 3, Deng Ming-quan2, 3, Luo Xin4, Yao Run-si4, Gao Zhi5, Wang Yi-fei1, 2

【Setting】

1Biomedicine Research and Development Centre of Jinan University, Guangzhou  510632, Guangdong Province, China;

2Guangzhou (Jinan)-Hong Kong Joint Laboratory of Cell Engineering, Guangzhou  510632, Guangdong Province, China; 3Asia-Pacific Stem Cell Research Centre Co., Ltd., Hong Kong  999077, China; 4Department of Obstetrics and Gynecology, First Affiliated Hospital of Jinan University, Guangzhou  510632, Guangdong Province, China; 5Department of Bioengineering, Clemson University, Clemson, SC 29634-0905, USA

【Found】

the Foundation of Guangzhou (Jinan)-Hong Kong Joint Laboratory*; International Cooperation Exchange for Science and Technology*

【Abstract】

BACKGROUND: Culture condition, isolation method and efficiency are different in reported human umbilical cord-derived mesenchymal stem cells, which lack of unified identification standards. Therefore, it is necessary to establish a high-efficiency and economical culture system for human umbilical cord-derived mesenchymal stem cells (hUCMSCs).

OBJECTIVE: To isolate hUCMSCs and induced differentiate into adipocytes and osteblasts.

METHODS: The hUCMSCs were isolated form human umbilical cord by tissue adherence and digested with collagenase. The morphology, proliferation and immunophenotype of the 3rd passage cells were analyzed, and then cells were induced to osteogenic and adipogenic differentiation in vitro.

RESULTS AND CONCLUSION: The hUCMSCs isolated from human umbilical cord by tissue adherence and digested with collagenase could be cultured and proliferated in vitro. Flow cytometry analysis revealed that the hUCMSCs were positive for CD29, CD44, CD59, CD105, but were negative for CD40, CD86 and HLA-DR. These cells could be induced to differentiate into adipocytes and osteblasts under proper inducing conditions. The hUCMSCs retained the appearance and phenotype even after being expanded more than 40 passages in vitro. This confirmed that the existence of MSCs in human umbilical cord and they had the capacity of differentiating into adipocytes and osteblasts.

【JPG pictures】

6 figures click

Figure 1  Primary culture of human umbilical cord-derived mesenchymal stem cells at 10 days after culture  (×100)
Figure 2  80% confluence of human umbilical cord-derived mesenchymal stem cells at Primary culture (×40)
Figure 3  Growth curve of the 3rd passage of human umbilical cord-derived mesenchymal stem cells
Figure 4  Antigen expression of the 3rd passage of human umbilical cord-derived mesenchymal stem cells
Figure 5  Oil red O staining of adipocytes (×200)
Figure 6  Alkaline phophatase staining of osteblasts (×40)

【Full text】